TLR4 gene expression in pig populations and its association with resistance to Escherichia coli F18.
TLR4 gene expression in pig populations and its association with resistance to Escherichia coli F18.
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DOI：
10.4238/2013.july.30.1
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发表时间：
2013-07
期刊：
影响因子：
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通讯作者：
J. Wang;Z. Pan;X. Zheng;Z. C. Wu;X. Su;G. Zhu;X. G. Huang;S. L. Wu;W. Bao
中科院分区：
文献类型：
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作者：
J. Wang;Z. Pan;X. Zheng;Z. C. Wu;X. Su;G. Zhu;X. G. Huang;S. L. Wu;W. Bao
TLR4是细菌脂多糖的主要识别受体，在天然免疫和获得性免疫中发挥重要作用。本研究利用实时荧光定量聚合酶链式反应技术，分析了TLR4基因在4个猪群(耐F18的苏台猪、敏感的苏泰猪、大白猪、眉山猪)中的组织表达谱和差异表达，以确定TLR4基因在抗F18中的作用。我们发现TLR4在4个种群中表达一致，在免疫组织中表达水平相对较高，在肺中表达水平最高。总体来看，TLR4在大肠杆菌F18敏感个体中的表达水平最高，其次是耐F18菌株、大白菌和梅山。在脾、肺、肾、淋巴结和胸腺中，对F18敏感的菌株TLR4的表达显著高于其他3个种群；耐F18的苏台、大白鼠和梅山的TLR4表达差异不显著。此外，基因本体论和通路分析表明，TLR4参与了炎症反应。我们发现猪TLR4在每个品种中具有一致的组织特异性，TLR4基因表达下调与断奶仔猪对E.ColiF18的抗性有关。
TLR4 is the main recognition receptor of bacterial lipopolysaccharides, which play an important role in innate and adaptive immunity. We used real-time PCR to analyze the tissue expression profile and differential expression of TLR4 in 4 pig populations (Escherichia coli F18-resistant Sutai, E. coli F18-sensitive Sutai, Large White, Meishan), in order to determine the role that the TLR4 gene plays in resistance to E. coli F18. We found that TLR4 expressed consistently in the 4 populations, with relatively high levels in immune tissues and the highest level in the lung. Generally, the expression of TLR4 in E. coli F18-sensitive individuals was the highest, followed by that in E. coli F18-resistant, Large White and Meishan. In the spleen, lung, kidney, lymph nodes, and thymus gland, TLR4 expression is significantly higher in the E. coli F18-sensitive than in the other 3 populations; there were no significant differences among E. coli F18-resistant Sutai, Large White, and Meishan. In addition, Gene Ontology and pathway analysis showed that TLR4 takes part in the inflammatory response. We found that porcine TLR4 has consistent tissue specificity in each breed, and downregulation of expression of the TLR4 gene is related to resistance to E. coli F18 in weaning piglets.