Developmental effects related to angiogenesis and osteogenic differentiation in Salmon larvae continuously exposed to dioxin-like 3,3′,4,4′-tetrachlorobiphenyl (congener 77)
Developmental effects related to angiogenesis and osteogenic differentiation in Salmon larvae continuously exposed to dioxin-like 3,3′,4,4′-tetrachlorobiphenyl (congener 77)
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DOI：
10.1016/j.aquatox.2011.09.005
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发表时间：
2011-10-01
影响因子：
4.5
通讯作者：
Arukwe, Augustine
中科院分区：
文献类型：
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作者：
Olufsen, Marianne;Arukwe, Augustine
研究了二恶英类3，3‘，4，4’-四氯联苯(PCB77)对鲑鱼早期发育过程中与血管生成和成骨相关的发育效应的影响。将幼虫保存在6℃下，从卵期或卵黄囊期(716dd)开始连续暴露于水性多氯联苯-77(1或10 ng/L)，总持续时间为50d(或300dd)。用实时荧光定量聚合酶链式反应(Real-Time-PCR)对548、632、674和716d的幼虫总RNA进行基因转录分析。研究骨形态发生蛋白(BMP2和BMP4)、转化生长因子-β(TGF-β)、雌激素受体(ERα和ERβ)、Runx2、Sox9和2型胶原蛋白α1(Col2a1)和血管内皮生长因子(VEGF)基因。对血管内皮生长因子基因转录的影响与心率、心律失常和贫血的观察有关，显示了对血管系统发育的影响。阿利沙林红染色和骨化骨结构定量显示，PCB-77产生浓度依赖的成骨组织形成率增加。PCB77诱导COL2al和Runx2转录增加，随后分别诱导软骨形成和成骨。转化生长因子-β基因的转录与ER-β的转录相关。PCB77对AhR基因的转录产物有不同程度的调节作用，且这种调节作用与浓度和幼虫年龄有关。PCB77对血管系统的破坏表现为心脏水肿、贫血和心律失常，以及早年时VEGF基因转录水平的降低。总体而言，我们的数据表明，PCB-77产生了与血管生成、成骨分化和血管系统发育中断相关的发育效应。(C)2011爱思唯尔B.V.保留所有权利。
We have studied the effects of dioxin-like 3,3',4,4'-tetrachlorobiphenyl (PCB-77) on developmental effects related to angiogenesis and osteogenesis during early life-stages of salmon. Larvae were kept at 6 degrees C and continuously exposed to waterborne PCB-77 (1 or 10 ng/L) initiated at the egg stage or 416-day degrees (dd) and throughout yolk-sac stage (716 dd) and for a total duration of 50 days (or 300 dd). Gene transcription analysis was performed on whole larvae total RNA at 548, 632, 674 and 716 dd using real-time PCR. Bone morphogenetic protein (bmp2 and bmp4), transforming growth factor beta (TGF-beta), estrogen receptors (ER alpha and ER beta), runx2, sox9 and collagen type 2 alpha 1 (col2a1) and vascular endothelial growth factor (VEGF) genes were studied. Effect on VEGF gene transcription was related to observation of heart rate, arrhythmia and anemia, demonstrating effects on vascular system development. Alizarine-red staining and quantification of ossified bone structures showed that PCB-77 produced concentration-dependent increases in the rate of osteogenic tissue formation. PCB-77 produced increases in col2al and runx2 transcription with subsequent induction of chondrogenesis and osteogenesis, respectively. The transcription of TGF-beta gene was associated with ER beta transcription. Transcripts of AhR gene battery were differentially modulated by PCB-77 and these effects were dependent on concentration and larval age. Evidence of vascular system disruption by PCB-77 was observed as cardiac edema, anemia and arrhythmia in exposed individuals and as decreased level of VEGF gene transcription at early age. In general, our data indicate that PCB-77 produced developmental effects related to angiogenesis and osteogenic differentiation and disruption of vascular system development. (C) 2011 Elsevier B.V. All rights reserved.