Diversity of cellular physiology and morphology of Purkinje cells in the adult zebrafish cerebellum
Diversity of cellular physiology and morphology of Purkinje cells in the adult zebrafish cerebellum
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DOI：
10.1002/cne.25435
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发表时间：
2023-02-01
影响因子：
2.5
通讯作者：
Han，Victor Z. Z.
中科院分区：
文献类型：
--
作者：
Magnus，Gerhard;Xing，Junling;Han，Victor Z. Z.
这项研究旨在探索成年斑马鱼小脑的功能电路，重点是浦肯野细胞，并在切片制备中使用全细胞贴片记录和单细胞标记。在生理学表征之后，记录的单细胞被标记用于形态学鉴定。结果发现，斑马鱼的浦肯野细胞是惊人的多样性。根据其生理和形态特征，可将其分为三种亚型：I型，尖形锋细胞，仅发射窄的Na+锋（持续时间<3 ms），并且具有单个初级枝晶，其乔木限于远端分子层; II型，国外锋细胞，它会释放大量的钙离子（持续时间为5-7 ms），并且在内部分子层中具有有限分支的初级枝晶，然后进一步辐射整个分子层; III型，一个非常宽的棘细胞，它发射非常宽的Ca 2+棘波（持续时间≥10 ms），并具有一个密集的近端树突状乔木，要么局限于内部分子层（IIIa型），要么辐射整个分子层（IIIb型）。这些浦肯野细胞对平行纤维激活的反应的分级配对脉冲易化和攀爬纤维激活的全或无配对脉冲抑制在很大程度上与哺乳动物的报告相似。这些浦肯野细胞的标记轴突终末局部结束，如报道的幼斑马鱼。本研究提供的证据表明，相应的功能电路和信息处理不同于哺乳动物小脑中已经确立的功能。
This study was designed to explore the functional circuitry of the adult zebrafish cerebellum, focusing on its Purkinje cells and using whole‐cell patch recordings and single cell labeling in slice preparations. Following physiological characterizations, the recorded single cells were labeled for morphological identification. It was found that the zebrafish Purkinje cells are surprisingly diverse. Based on their physiology and morphology, they can be classified into at least three subtypes: Type I, anarrow spike cell, which fires only narrow Na+spikes (<3 ms in duration), and has a single primary dendrite with an arbor restricted to the distal molecular layer; Type II, abroad spike cell, which fires broad Ca2+spikes (5–7 ms in duration) and has a primary dendrite with limited branching in the inner molecular layer and then further radiates throughout the molecular layer; and Type III, avery broad spike cell, which fires very broad Ca2+spikes (≥10 ms in duration) and has a dense proximal dendritic arbor that is either restricted to the inner molecular layer (Type IIIa), or radiates throughout the entire molecular layer (Type IIIb). The graded paired‐pulse facilitation of these Purkinje cells’ responses to parallel fiber activations and the all‐or‐none, paired‐pulse depression of climbing fiber activation are largely similar to those reported for mammals. The labeled axon terminals of these Purkinje cells end locally, as reported for larval zebrafish. The present study provides evidence that the corresponding functional circuitry and information processing differ from what has been well‐established in the mammalian cerebellum.