Micromorphology of Resin-Dentin Interfaces Using One-Bottle Etch&Rinse and Self-Etching Adhesive Systems on Laser-Treated Dentin Surfaces: A Confocal Laser Scanning Microscope Analysis
Micromorphology of Resin-Dentin Interfaces Using One-Bottle Etch&Rinse and Self-Etching Adhesive Systems on Laser-Treated Dentin Surfaces: A Confocal Laser Scanning Microscope Analysis
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DOI：
10.1002/lsm.20945
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发表时间：
2010-09-01
影响因子：
2.4
通讯作者：
Giannini, Marcelo
中科院分区：
文献类型：
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作者：
de Oliveira, Marcelo Tavares;Galvao Arrais, Cesar Augusto;Giannini, Marcelo
背景与目的：本研究评价了三种粘接系统（AS）在Er：YAG激光处理牙本质表面上产生的混合层（HL）形态，使用两种照射参数。研究设计：36颗人类第三磨牙的咬合平坦牙本质表面根据以下AS被分为9组（n = 4）：一瓶蚀刻和冲洗单键Plus（3 M ESPE），两步Clearfil保护键（Kuraray），和多合一S3键（Kuraray）自蚀刻，用罗丹明B或异硫氰酸荧光素葡聚糖标记，并应用于用Er：YAG激光以120（38.7 J/cm（2））或200 mJ/脉冲（64.5 J/cm（2））照射的牙本质表面，或应用于未处理的牙本质表面（对照组）。在MI后将AS光活化，并用树脂复合物Z250（3 M ESPE）修复粘结表面。在植物油中储存24小时后，将修复的牙齿垂直地连续切片成1 mm厚的平板，其具有用共聚焦激光显微镜（CLSM-LSM 510 Meta）分析的粘附界面。CLSM图像记录在荧光模式下从三个不同的区域沿着每个粘接interfaces.Results：不均匀的HL激光照射牙本质表面上创建无论激光照射协议所有AS，而规则和均匀的HL观察到在对照组。由于树脂渗入牙本质微裂隙，在HL内发现“妊娠纹”样红线，这主要在200 mJ/脉冲组中观察到，与AS无关。差树脂渗透到管周牙本质中观察到在大多数地区的所有AS的激光照射牙本质上创建的粘合剂接口，导致薄树脂标签既没有漏斗形的形态，也没有横向树脂projections.Conclusion：激光照射牙本质表面在120或200毫焦耳/脉冲导致HL和树脂标签的形态学变化的所有AS在研究中评估。激光外科医学42：662-670，2010年。(C)2010 Wiley-Liss，Inc.
Background and Objectives: This study evaluated the hybrid layer (HL) morphology created by three adhesive systems (AS) on dentin surfaces treated with Er:YAG laser using two irradiation parameters.Study Design: Occlusal flat dentin surfaces of 36 human third molars were assigned into nine groups (n = 4) according to the following ASs: one bottle etch&rinse Single Bond Plus (3M ESPE), two-step Clearfil Protect Bond (Kuraray), and all-in-one S3 Bond (Kuraray) self-etching, which were labeled with rhodamine B or fluorescein isothiocyanate dextran and were applied to dentin surfaces that were irradiated with Er:YAG laser at either 120 (38.7 J/cm(2)) or 200 mJ/pulse (64.5 J/cm(2)), or were applied to untreated dentin surfaces (control group). The ASs were light-activated following MI and the bonded surfaces were restored with resin composite Z250 (3M ESPE). After 24 hours of storage in vegetable oil, the restored teeth were vertically, serially sectioned into 1-mm thick slabs, which had the adhesive interfaces analyzed with confocal laser microscope (CLSM-LSM 510 Meta). CLSM images were recorded in the fluorescent mode from three different regions along each bonded interface.Results: Non-uniform HL was created on laser-irradiated dentin surfaces regardless of laser irradiation protocol for all AS, while regular and uniform HL was observed in the control groups. "Stretch mark"-like red lines were found within the HL as a result of resin infiltration into dentin microfissures, which were predominantly observed in 200 mJ/pulse groups regardless of AS. Poor resin infiltration into peritubular dentin was observed in most regions of adhesive interfaces created by all ASs on laser-irradiated dentin, resulting in thin resin tags with neither funnel-shaped morphology nor lateral resin projections.Conclusion: Laser irradiation of dentin surfaces at 120 or 200 mJ/pulse resulted in morphological changes in HL and resin tags for all ASs evaluated in the study. Lasers Surg. Med. 42:662-670, 2010. (C) 2010 Wiley-Liss, Inc.