In Vitro and In Vivo Effectiveness of an Innovative Silver-Copper Nanoparticle Coating of Catheters To Prevent Methicillin-Resistant Staphylococcus aureus Infection
In Vitro and In Vivo Effectiveness of an Innovative Silver-Copper Nanoparticle Coating of Catheters To Prevent Methicillin-Resistant Staphylococcus aureus Infection
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DOI：
10.1128/aac.00959-16
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发表时间：
2016-09-01
影响因子：
4.9
通讯作者：
Bizzini, Alain
中科院分区：
文献类型：
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作者：
Ballo, Myriam K. S.;Rtimi, Sami;Bizzini, Alain
在这项研究中，研究了银/铜（Ag/Cu）涂层导管在体外和体内预防耐甲氧西林金黄色葡萄球菌（MRSA）感染的有效性。银和铜溅射（67/33%原子比）聚氨酯导管上的直流磁控溅射。在体外，将Ag/Cu涂层和未涂层导管浸入磷酸盐缓冲盐水（PBS）或大鼠血浆中，并暴露于10（4）至10（8）CFU/ml的MRSA ATCC 43300。在体内，Ag/Cu涂层和未涂层的导管被放置在大鼠的颈静脉。之后立即在尾静脉中接种MRSA（10（7）CFU/ml）。48 h后取出导管并培养。在体外，在PBS中预孵育并暴露于10（4）至10（7）CFU/ml的Ag/Cu涂层导管与未涂层导管（50至100%定植; P
In this study, silver/copper (Ag/Cu)-coated catheters were investigated for their efficacy in preventing methicillin-resistant Staphylococcus aureus (MRSA) infection in vitro and in vivo. Ag and Cu were sputtered (67/33% atomic ratio) on polyurethane catheters by direct-current magnetron sputtering. In vitro, Ag/Cu-coated and uncoated catheters were immersed in phosphatebuffered saline (PBS) or rat plasma and exposed to MRSA ATCC 43300 at 10(4) to 10(8) CFU/ml. In vivo, Ag/Cu-coated and uncoated catheters were placed in the jugular vein of rats. Directly after, MRSA (10(7) CFU/ml) was inoculated in the tail vein. Catheters were removed 48 h later and cultured. In vitro, Ag/Cu-coated catheters preincubated in PBS and exposed to 10(4) to 10(7) CFU/ml prevented the adherence of MRSA (0 to 12% colonization) compared to uncoated catheters (50 to 100% colonization; P