Nitrogen redistribution and its relationship with the expression of GmATG8c during seed filling in soybean
Nitrogen redistribution and its relationship with the expression of GmATG8c during seed filling in soybean
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DOI：
10.1016/j.jplph.2016.01.007
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发表时间：
2016-03-15
影响因子：
4.3
通讯作者：
Zheng, Shao-Hui
中科院分区：
文献类型：
--
作者：
Islam, Md. Matiul;Ishibashi, Yushi;Zheng, Shao-Hui
众所周知，大豆（Glycine max [L.]）营养器官中的一些氮在种子灌浆期间被重新分配到种子中。梅里尔）。这种再分配被认为影响大豆的种子产量。但是，氮素何时从营养体转移到种子中，以及氮素再分配与叶片衰老的关系尚不清楚。大豆品种Fukuyutaka于2014年7月22日至10月31日在日本佐贺大学的实验田中种植。在第一个花期（R1）后，每周收集植物样品，并将其分成叶、叶柄、茎、荚壳和种子。测定了植株各部位的氮素浓度。提供新鲜叶片样品用于测定可溶性蛋白和自噬基因GmATG8c表达。营养器官中氮素积累量在播后60天达到最高值，73天开始下降。这种减少被认为是氮从营养部分重新分配到种子的结果。据估计，氮从营养部分向种子的移动发生在大约73 DAS（R6）。在此阶段，叶片SPAD值，叶片氮，可溶性蛋白质浓度开始同时下降，表明叶片衰老的开始。此外，自噬基因GmATG8c在叶片中的表达急剧增加，从73至85 DAS，这是氮再分配的持续时间。结果表明，氮素从营养器官向种子的再分配可能是叶片衰老的启动因子之一，自噬基因GmATG8c与这一过程有关。(C)2016年爱思唯尔有限公司。All rights reserved.
It is well known that some nitrogen in the vegetative organs is redistributed to the seeds during seed filling in soybean (Glycine max [L.] Merrill). This redistribution is considered to affect the seed yield of soybean. However, it is still not clear when the nitrogen moves from the vegetative part to the seeds, and the relationship between nitrogen redistribution and leaf senescence has not been clarified. The soybean variety Fukuyutaka was grown in the experimental field of Saga University, Japan from 22 July to 31 October, 2014. After the first flower stage (R1), the plant samples were collected weekly and were separated into leaf, petiole, stem, podshell and seed. The nitrogen concentrations in each plant part were determined. Fresh leaf samples were provided for the determination of soluble protein and autophagy gene GmATG8c expression. The nitrogen that accumulated in the vegetative parts reached its highest level at 60 days after sowing (DAS), then began to decrease at 73 DAS (R6). This decrease is considered to be the consequence of nitrogen redistribution from the vegetative parts to the seeds. The movement of nitrogen from the vegetative parts to the seeds was estimated to occur at around 73 DAS (R6). At this stage, leaf SPAD values, leaf nitrogen, and soluble protein concentrations began to decrease simultaneously, suggesting the onset of leaf senescence. Furthermore, the expression of the autophagy gene GmATG8c in the leaves increased dramatically from 73 to 85 DAS, which is the duration of nitrogen redistribution. The results suggest that the nitrogen redistribution from the vegetative parts to the seeds could be one of the initiating factors of leaf senescence, and the autophagy gene GmATG8c was associated with this process. (C) 2016 Elsevier GmbH. All rights reserved.