Development of a flexible and specific gene delivery system for production of murine tumor models
Development of a flexible and specific gene delivery system for production of murine tumor models
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DOI：
10.1038/sj.onc.1203087
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发表时间：
1999-09-20
期刊：
影响因子：
8
通讯作者：
Varmus, HE
中科院分区：
文献类型：
--
作者：
Fisher, GH;Orsulic, S;Varmus, HE
为了建立人类癌症模型，Ne等人在转基因小鼠中表达了禽类逆转录病毒受体TVA，在各种哺乳动物启动子下，从而使小鼠容易感染禽流感病毒衍生的基因载体。基于TVA的逆转录病毒基因转移比目前的小鼠人类癌症模型更具优势。单个转基因小鼠品系可以单独或组合用于评估多个遗传损伤。此外，突变基因是通过体细胞导入动物体内的，就像大多数自然产生的转化者所发生的那样。由于禽类病毒载体只在禽类细胞中复制，受感染的转基因小鼠细胞中的病毒受体仍可用于不同ASLV载体的多轮感染。我们讨论了使用重组禽类逆转录病毒与TVA转基因小鼠建立肿瘤模型的理论和实践方面。
To develop models of human cancer ne have expressed the avian retroviral receptor, TVA, under a variety of mammalian promoters in transgenic mice, thus rendering mice susceptible to infection with avian leukosis virus-derived gene vectors. TVA-based retroviral gene transfer offers advantages ol er current murine models of human cancer. A single transgenic mouse line can be used to evaluate multiple genetic lesions, individually and in combination. Furthermore, mutant genes are introduced somatically into animals, as occurs in the majority of naturally occurring turners. Because the avian viral vectors replicate only in avian cells, the viral receptor in infected transgenic mouse cells remains available for multiple rounds of infection with different ASLV vectors. We discuss the theoretical and practical aspects of using recombinant avian retroviruses with TVA transgenic mice to generate cancer models.