Phosphocreatine and ATP content in human single muscle fibres before and after maximum dynamic exercise
Phosphocreatine and ATP content in human single muscle fibres before and after maximum dynamic exercise
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DOI：
10.1007/s004240100552
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发表时间：
2001-06-01
影响因子：
4.5
通讯作者：
Sargeant, AJ
中科院分区：
文献类型：
--
作者：
Karatzaferi, C;de Haan, A;Sargeant, AJ
本文研究了人骨骼肌单纤维高能磷酸盐水平在短期极限高负荷运动后的恢复。三名男性志愿者在等速自行车测功仪上最大运动25 s。在休息时、运动后即刻和恢复后1.5 min采集股外侧肌的肌肉活检样本。受试者还在第一次运动后1.5分钟进行了第二次运动。单个肌纤维进行解剖，其特征在于，并分配到四个组之一，根据其肌球蛋白重链（MyHC）亚型的内容，即，I型，IIA型，IIAX和IIXa（后两组含有小于或大于50%的IIX MyHC）。分析纤维的5 ' -三磷酸腺苷（ATP）、5 ' -单磷酸肌苷（IMP）、磷酸肌酸（PCr）和肌酸（Cr）水平。Ⅰ型纤维的Clr含量低于Ⅱ型纤维（P
The recovery of high-energy phosphate levels in single human skeletal muscle fibres following shortterm maximal tall-out) exercise was investigated. Three male volunteers exercised maximally for 25 s on an isokinetic cycling ergometer. Muscle biopsy samples from the vastus lateralis were collected at rest, immediately postexercise and at 1.5 min of recovery. The subjects also per formed a second exercise bout 1.5 min after the first, on a separate occasion. Single muscle fibres were dissected, characterized and assigned to one of four groups according to their myosin heavy chain (MyHC) isoform content; namely, type I, IIA, IIAx and IIXa (the latter two groups containing either less or more than 50% IIX MyHC). Fibres were analysed for adenosine 5 ' -triphosphate (ATP), inosine-5 ' -monophosphate (IMP), phosphocreatine (PCr) and creatine (Cr) levels. Type I fibres had a lower Clr content than type II fibres (P