Coding function for non-coding RNA in plants—insights from miRNA encoded peptide (miPEP)
Coding function for non-coding RNA in plants—insights from miRNA encoded peptide (miPEP)
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DOI：
10.1007/s11427-015-4854-z
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发表时间：
2015-04
期刊：
影响因子：
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通讯作者：
Long Wang;Jia-Wei Wang
中科院分区：
文献类型：
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作者：
Long Wang;Jia-Wei Wang
非编码RNA（Non-coding RNA，ncRNA）是指大量不同的多聚腺苷酸化或非多聚腺苷酸化的RNA转录本，具有低蛋白质编码潜力。一般而言，看家ncRNA（例如，核糖体RNA和转移RNA）、microRNA的初级转录物（miRNA）和植物特异性RNA聚合酶V（Pol V）依赖的ncRNA转录物也属于该定义。下一代测序的应用导致在植物中鉴定超过30，000种推定的ncRNA [1]。虽然一些ncRNA可能是转录噪音或RNA加工和降解的副产物，但越来越多的证据表明它们中的很大一部分是功能性的，并在发育过程中发挥各种调节活性。最近的研究揭示了长ncRNA的作用（lncRNA）在调节基因表达中的作用。IPS 1（磷酸饥饿诱导1）编码一种具有短基序的ncRNA，该基序与miR 399的序列高度互补，除了在预期的miR 399切割位点处的错配环。错配环使IPS 1能够作为miRNA靶标模拟物（不可降解的miRNA靶标）发挥作用并螯合miR 399活性[2]。Pol V依赖性ncRNA转录物涉及RNA指导的DNA甲基化，其间接导致基因沉默[3]。lncRNA还可以通过募集
Non-coding RNAs (ncRNAs) refer to a large and diverse collection of polyadenylated or non-polyadenylated RNA transcripts with low protein coding potential. In general, the house-keeping ncRNAs (eg, ribosomal RNAs and transfer RNAs), primary transcripts of microRNAs (miRNAs) and plant specific RNA polymerase V (Pol V)-dependent ncRNA transcripts also fall into this definition. The application of next-generation sequencing leads to identification of over 30,000 putative ncRNAs in plants [1]. While it is possible that some ncRNAs are transcription noises or the by-products of RNA processing and degradation, growing lines of evidence suggest that a large fraction of them are functional and play various regulatory activities during development.Recent studies have revealed the role of long ncRNAs (lncRNAs) in regulating gene expression. IPS1 (INDUCED BY PHOSPHATE STARVATION1) encodes a ncRNA with a short motif that is highly complementary to the sequence of miR399 except a mismatched loop at the expected miR399 cleavage site. The mismatch loop enables IPS1 to function as a miRNA target mimicry (non-degradable miRNA target) and sequestrate miR399 activity [2]. Pol V-dependent ncRNA transcripts have been implicated in RNA-directed DNA methylation, which indirectly causes gene silencing [3]. lncRNA can also regulate gene expression by recruiting