REQUIREMENT OF A CRITICAL PERIOD OF TRANSCRIPTION FOR INDUCTION OF A LATE-PHASE OF LTP
REQUIREMENT OF A CRITICAL PERIOD OF TRANSCRIPTION FOR INDUCTION OF A LATE-PHASE OF LTP
复制标题
DOI：
10.1126/science.8066450
复制
发表时间：
1994-08-19
期刊：
影响因子：
56.9
通讯作者：
KANDEL, ER
中科院分区：
文献类型：
--
作者：
NGUYEN, PV;ABEL, T;KANDEL, ER
重复的高频率刺激在海马脑片的CA 1区诱导长时程增强（LTP），持续长达8小时，在完整的动物中持续数天。这种长时间的过程使得LTP成为哺乳动物大脑中某些形式的长期记忆的有吸引力的模型。完整动物的长期记忆的一个标志是需要转录，因此在此研究了LTP的晚期阶段（L-LTP）是否需要转录。使用不同的抑制剂，在大鼠海马切片中发现，当在强直刺激后立即或在应用cAMP期间阻断转录时，L-LTP [由强直刺激或应用环磷酸腺苷（cAMP）类似物Sp-cAMPS（SP-环腺苷3 '，5'-单硫代磷酸盐）产生]的诱导被选择性地阻止。与行为记忆一样，这种转录要求有一个关键的时间窗口，因此，CA 1区LTP的晚期阶段需要在关键时期进行转录，也许是因为cAMP诱导型基因必须在此期间表达。
Repeated high-frequency trains of stimuli induce long-term potentiation (LTP) in the CA1 region that persists for up to 8 hours in hippocampal slices and for days in intact animals. This long time course has made LTP an attractive model for certain forms of long-term memory in the mammalian brain. A hallmark of long-term memory in the intact animal is a requirement for transcription, and thus whether the late phase of LTP (L-LTP) requires transcription was investigated here. With the use of different inhibitors, it was found in rat hippocampal slices that the induction of L-LTP [produced either by tetanic stimulation or by application of the cyclic adenosine monophosphate (cAMP) analog Sp-cAMPS (Sp-cyclic adenosine 3',5'-monophosphorothioate)] was selectively prevented when transcription was blocked immediately after tetanization or during application of cAMP. As with behavioral memory, this requirement for transcription had a critical time window, Thus, the late phase of LTP in the CA1 region requires transcription during a critical period, perhaps because cAMP-inducible genes must be expressed during this period.