Rice bran proteins and their hydrolysates modulate cholesterol metabolism in mice on hypercholesterolemic diets
Rice bran proteins and their hydrolysates modulate cholesterol metabolism in mice on hypercholesterolemic diets
复制标题
米糠蛋白及其水解产物调节高胆固醇血症饮食小鼠的胆固醇代谢
DOI：
10.1039/c6fo00044d
复制
发表时间：
2016
期刊：
影响因子：
6.1
通讯作者：
Sun Baoguo
中科院分区：
文献类型：
--
作者：
Zhang Huijuan;Wang Jing;Liu Yingli;Gong Lingxiao;Sun Baoguo
用高脂饲料喂养小鼠4周，测定了脱脂米糠蛋白(DRBP)、新鲜米糠蛋白(FRBP)、脱脂米糠蛋白水解物(DRBPH)和新鲜米糠蛋白水解物(FRBPH)的降血脂特性。FRBPH饮食组大鼠极低密度脂蛋白胆固醇(VLDL-C)、低密度脂蛋白胆固醇(LDL-C)含量和肝脏总胆固醇含量降低，粪便总胆固醇和总胆汁酸(TBA)含量升高。肝脏胆固醇生物合成相关基因HMG-Coar和SREBP-2在FRBPH饮食组的表达水平最低。HMG-Coar基因表达水平与肝脏TG含量呈显著正相关(r=0.82，P<；0.05)。与胆汁酸生物合成和胆固醇外流相关的基因、细胞色素P7A1、ABCA1和PPARγ在各试验组均表达上调。结果表明，FRBPH通过增加粪便类固醇排泄量和胆汁酸合成及胆固醇外流相关基因的表达水平，下调胆固醇生物合成相关基因的表达水平来调节高脂高胆固醇饮食小鼠的胆固醇代谢。
The hypolipidemic properties of defatted rice bran protein (DRBP), fresh rice bran protein (FRBP), DRBP hydrolysates (DRBPH), and FRBP hydrolysates (FRBPH) were determined in mice on high fat diets for four weeks. Very low-density lipoprotein cholesterol (VLDL-C), low-density lipoprotein cholesterol (LDL-C) contents, and the hepatic total cholesterol content were reduced while fecal total cholesterol and total bile acid (TBA) contents were increased in the FRBPH diet group. The expression levels of hepatic genes for cholesterol biosynthesis HMG-CoAR and SREBP-2 were lowest in the FRBPH diet group. The mRNA level of HMG-CoAR was significantly positively correlated with the hepatic TG content (r = 0.82, P < 0.05). The mRNA levels of genes related to bile acid biosynthesis and cholesterol efflux, CYP7A1, ABCA1, and PPARγ were up-regulated in all test groups. The results suggest that FRBPH regulates cholesterol metabolism in mice fed the high fat and cholesterol diet by increasing fecal steroid excretion and expression levels of genes related to bile acid synthesis and cholesterol efflux, and the down-regulation of the expression levels of genes related to cholesterol biosynthesis.