Deletion of a Histone Acetyltransferase Leads to the Pleiotropic Activation of Natural Products in Metarhizium robertsii
Deletion of a Histone Acetyltransferase Leads to the Pleiotropic Activation of Natural Products in Metarhizium robertsii
复制标题
组蛋白乙酰转移酶的缺失导致罗伯氏绿僵菌天然产物的多效性激活
DOI：
10.1021/acs.orglett.7b00476
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发表时间：
2017-04-07
期刊：
影响因子：
5.2
通讯作者：
Yin, Wen-Bing
中科院分区：
文献类型：
--
作者：
Fan, Aili;Mi, Wubin;Yin, Wen-Bing
组蛋白去乙酰化通常会降低生物体中的基因表达。通过昆虫病原真菌罗伯茨绿僵菌表观遗传调控基因的全基因组缺失，在组蛋白乙酰转移酶（HAT）基因Hat 1被破坏后，发现了孤儿次级代谢物基因的意外激活。这导致了11个新的天然产物的表征，包括8个异香豆素衍生物meromutides A-H和两个非核糖体肽meromutides A和B。因此，破坏HAT代表了从真菌中挖掘化学多样性的新方法。
Histone deacetylation normally decreases the gene expression in organisms. By genome-wide deletions of epigenetic regulators in entomopathogenic fungus Metarhizium robertsii unexpected activations of orphan secondary metabolite genes have been found upon the disruption of a histone acetyltransferase (HAT) gene Hat1. This led to the characterization of 11 new natural products, including eight isocoumarin derivatives meromusides A-H and two nonribosomal peptides meromutides A and B. Therefore, disruption of HAT represents a new approach to mine chemical diversity from fungi.