Early Development of Medaka Oryzias latipes Muscles as Revealed by Transgenic Approaches Using Embryonic and Larval Types of Myosin Heavy Chain Genes
Early Development of Medaka Oryzias latipes Muscles as Revealed by Transgenic Approaches Using Embryonic and Larval Types of Myosin Heavy Chain Genes
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DOI：
10.1002/dvdy.22298
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发表时间：
2010-06-01
影响因子：
2.5
通讯作者：
Watabe, Shugo
中科院分区：
文献类型：
--
作者：
Ono, Yosuke;Kinoshita, Shigeharu;Watabe, Shugo
我们克隆了三个全长cDNA编码肌球蛋白重链（MYH）先前发现在青鳉胚胎或幼虫表达。根据cDNA序列信息，将青鳉MYH基因mMYH（emb 1）、mMYHL（L1）和mMYH（L2）定位于染色体上。利用编码绿色或红色荧光蛋白的基因与mMYH的5 '-侧翼区连接进行的体内启动子分析表明，最早在胚胎中表达但在系统发育分析中属于成人型的快速型mMYH（emb 1）的转录本位于水平肌隔中。另一方面，胚胎快型mMYH（L1）和mMYH（L2）在整个肌节中表达。有趣的是，表达mMYH（emb 1）的细胞与engrailed和cyclopamine一起定位，cyclopamine阻断hedgehog信号传导，抑制mMYH（emb 1）表达以及水平肌间隔的形成，表明肌肉先锋细胞表达mMYH（emb 1）作为水平肌间隔形成的关键蛋白。发展动力学239：1807-1817，2010年。(C)2010 Wiley-Liss，Inc.
We cloned three full-length cDNAs encoding myosin heavy chains (MYHs) previously found to be expressed in embryos or larvae of medaka Oryzias latipes. Based on cDNA sequence information, the three medaka MYH genes, mMYH(emb1), mMYHL(L1) and mMYH(L2) were localized on the chromosomes. In vivo promoter assay using the gene encoding green or red fluorescent protein and linked to the 5'-flanking region of mMYH demonstrated that the transcripts of fast-type mMYH(emb1), first expressed in embryos but belonging to the adult type in phylogenetic analysis, were located in the horizontal myoseptum. On the other hand, embryonic fast-type mMYH(L1) and mMYH(L2) were expressed in the whole myotomes. Interestingly, cells expressing mMYH(emb1) were localized together with engrailed, and cyclopamine, which blocks hedgehog signaling, inhibited mMYH(emb1) expression as well as the formation of the horizontal myoseptum, suggesting that muscle pioneer cells express mMYH(emb1) as a key protein in the formation of the horizontal myoseptum. Developmental Dynamics 239:1807-1817, 2010. (C) 2010 Wiley-Liss, Inc.