Preventive effects of protopanaxadiol and protopanaxatriol ginsenosides on liver inflammation and apoptosis in hyperlipidemic apoE KO mice
Preventive effects of protopanaxadiol and protopanaxatriol ginsenosides on liver inflammation and apoptosis in hyperlipidemic apoE KO mice
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DOI：
10.1007/s12263-011-0245-7
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发表时间：
2012-04
影响因子：
3.5
通讯作者：
Soojeong Jang;Y. Lim;G. Valacchi;S. Sorn;Hyon Park;N. Park;Myoungsook Lee
中科院分区：
文献类型：
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作者：
Soojeong Jang;Y. Lim;G. Valacchi;S. Sorn;Hyon Park;N. Park;Myoungsook Lee
人参皂苷是人参的生物活性成分。Meyer，分为原人参二醇（PD）和原人参三醇（PT）。本研究的目的是评价不同比例的PD和PT组合对高脂血症apo E KO小鼠肝脏炎症和细胞凋亡的保护作用。R1（PD/PT = 1，高Rg 1和Rb 1）和R2（PD/PT = 2，高Re和Rd）提取物在第8周以100 mg/kg/d腹腔注射。R1和R2通过选择性升高HDL和降低总胆固醇（TC）和甘油三酯（TG）改善动脉粥样硬化指数。R1降低高脂血症小鼠血浆和肝组织脂质过氧化物（LPO）水平，R2降低血浆丙二醛（MDA）水平。R1和R2不仅能调节肝细胞内环氧合酶（考克斯）-2、IκB-α、磷酸化ERK 1/2和磷酸化SAPK/JNK的表达水平，还能显著阻断肝细胞内凋亡信号如caspase-8、-9以及PARP的裂解。PD和PT提取物的不同组合处理可能改善高脂血症apo E KO小鼠的肝脏炎症和细胞凋亡，这是动脉粥样硬化动物模型。
Ginsenosides, bioactive compounds ofPanax GinsengC.A. Meyer, are divided into protopanaxadiol (PD) and protopanaxtriol (PT). The aim of this study was to evaluate the protective effects of different PD and PT combination ratios on liver inflammation and apoptosis in hyperlipidemic apo E KO mice. R1 (PD/PT = 1, high Rg1and Rb1) and R2 (PD/PT = 2, high Re and Rd) extracts were intraperitoneally injected by 100 mg/kg/day at the 8th week. R1 and R2 improved atherogenic indices by increasing HDL and lowering total cholesterol (TC) and triacylglyceride (TG) selectively. R1 decreased lipid peroxides (LPO) level in plasma and liver tissue of hyperlipidemic mice, and R2 lowered plasma malondialdehyde(MDA) level. R1 and R2 not only regulated the expression of cyclooxygenase (COX)-2, IκB-α, phopho-ERK 1/2, and phopho-SAPK/JNK levels but also were significantly effective in blocking apoptotic signals, such as caspase-8, -9, as well as the cleavage of PARP in liver. Different combinational treatment of PD and PT extracts might ameliorate the liver inflammation and apoptosis in hyperlipidemic apo E KO mice, which is atherosclerotic animal model.