Molecular markers expressed in cultured and freshly isolated interstitial cells of Cajal
Molecular markers expressed in cultured and freshly isolated interstitial cells of Cajal
复制标题
DOI：
10.1152/ajpcell.2000.279.2.c529
复制
发表时间：
2000-08-01
影响因子：
5.5
通讯作者：
Horowitz, B
中科院分区：
文献类型：
--
作者：
Epperson, A;Hatton, WJ;Horowitz, B
位于胃肠道肌层内的是被称为Cajal间质细胞（ICC）的细胞网络。ICC对胃肠道运动的重要基本功能至关重要，如慢波起搏器活动的产生和传播以及肠神经系统调节输入的接收。我们开发了一种新的方法，从小鼠小肠和胃底新鲜分散的细胞制剂中鉴定和分离个体ICC，并确定差异转录表达。我们比较了从小鼠小肠分离的起搏器ICC （IC-MY）和参与胃底神经传递的ICC （IC-IM）的表达谱。我们还比较了ICC与平滑肌细胞（SMC）以及新鲜分离ICC与培养ICC之间的表达谱。培养的ICC表达平滑肌肌球蛋白，而新鲜分散的ICC则不表达。所有类型的细胞均表达毒蕈碱受体M-2和M-3，神经激肽受体NK1和NK3以及抑制受体VIP-1，而只有培养的ICC和SMC表达VIP-2。培养的和新鲜分散的IC-IM和IC-MY均表达可溶形式的干细胞因子，而胃底SMC仅表达膜结合形式的干细胞因子。
Located within the tunica muscularis of the gastrointestinal (GI) tract are networks of cells known as interstitial cells of Cajal (ICC). ICC are critical for important basic functions of GI motility such as generation and propagation of slow-wave pacemaker activity and reception of regulatory inputs from the enteric nervous system. We have developed a novel procedure to identify and isolate individual ICC from freshly dispersed cell preparations of the murine small intestine and gastric fundus and to determine differential transcriptional expression We have compared the expression profiles of pacemaker ICC isolated from the murine small intestine (IC-MY) and ICC involved in neurotransmission from the gastric fundus (IC-IM). We have also compared expression profiles between ICC and smooth muscle cells (SMC) and between freshly isolated ICC and cultured ICC. Cultured ICC express smooth muscle myosin, whereas freshly dispersed ICC do not. All cell types express muscarinic receptor types M-2 and M-3, neurokinin receptors NK1 and NK3, and inhibitory receptor VIP-1, whereas only cultured ICC and SMC express VIP-2. Both cultured and freshly dispersed IC-IM and IC-MY express the soluble form of stem cell factor, whereas SMC from the gastric fundus express only the membrane-bound form.