Programmed Control of Fluorescence Blinking Patterns based on Electron Transfer in DNA
Programmed Control of Fluorescence Blinking Patterns based on Electron Transfer in DNA
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DOI：
10.1002/chem.202203552
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发表时间：
2023-02-27
影响因子：
4.3
通讯作者：
Kawai，Kiyohiko
中科院分区：
文献类型：
--
作者：
Fan，Shuya;Takada，Tadao;Kawai，Kiyohiko
荧光成像利用荧光强度和发射波长的变化来同时分析多个目标。为了增加可以同时识别的目标数量，可以使用荧光闪烁作为附加参数。为了理解并最终控制眨眼，我们使用 DNA 作为平台来阐明导致眨眼的电子转移 (ET) 过程，直至速率常数。在固定的 ET 距离下，根据 DNA 平台的供体和受体单元之间的 DNA 序列，观察到各种闪烁模式。通过 ET 速率常数的组合成功地描述了眨眼模式。因此，可以通过调整 ET 来开发具有各种闪烁模式的分子。预计可同时分析的目标数量将随着闪烁模式数量的增加而增加。
Fluorescence imaging uses changes in the fluorescence intensity and emission wavelength to analyze multiple targets simultaneously. To increase the number of targets that can be identified simultaneously, fluorescence blinking can be used as an additional parameter. To understand and eventually control blinking, we used DNA as a platform to elucidate the processes of electron transfer (ET) leading to blinking, down to the rate constants. With a fixed ET distance, various blinking patterns were observed depending on the DNA sequence between the donor and acceptor units of the DNA platform. The blinking pattern was successfully described with a combination of ET rate constants. Therefore, molecules with various blinking patterns can be developed by tuning ET. It is expected that the number of targets that can be analyzed simultaneously will increase by the power of the number of blinking patterns.