Transforming Growth Factor 81 Positively Regulates Its Own Expression in Normal and Transformed Cells *
Transforming Growth Factor 81 Positively Regulates Its Own Expression in Normal and Transformed Cells *
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转化生长因子 81 积极调节其自身在正常和转化细胞中的表达 *
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
A. Roberts
中科院分区：
文献类型：
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作者：
E. Obberghen;N. Roche;K. Flanders;M. Sporn;A. Roberts
转化生长因子81 （TGF-81）调节几乎所有细胞类型的生长、分化或功能。我们现在报道TGF-81在培养的六种不同的正常细胞和转化细胞中增加其自身信息的稳态水平。在向细胞中加入TGF-81多肽后的3小时内，通过Northern blot分析可以检测到TGF-81 mRNA的积累，只要培养基中存在TGF81，增强的信息水平就会持续存在。在-10 PM TGF-81时，这种自动诱导是一半最大的，最大刺激对应于转录物水平的2-3倍增加。在正常大鼠肾细胞中，TGF-81 mRNA的升高是放线菌素d敏感的，并伴随着处理细胞培养基中TGF-81蛋白分泌的平行（-3倍）增加，这是通过使用特异性抗TGF-81抗体对生物合成标记的s6s标记的TGF-81进行免疫沉淀检测得到的。用表皮生长因子或血小板源性生长因子处理正常大鼠肾细胞也会导致TGF-81 mRNA增加（2-3倍），尽管表皮生长因子和TGF-81似乎通过不同的机制起作用，因为它们的联合作用大于相加作用。
Transforming growth factor 81 (TGF-81) regulates the growth, differentiation, or function of nearly all cell types. We now report hat TGF-81 increases steady-state levels of its own message in six different normal and transformed cells in culture. Accumulation of TGF-81 mRNA can be detected by Northern blot analysis within 3 h of addition of the peptide to cells, and enhanced message levels persist as long as TGF81 is present in the culture medium. This autoinduction is half-maximal at -10 PM TGF-81, and maximal stimulation corresponds to a 2-3-fold increase in transcript levels. In normal rat kidney cells, the rise in TGF-81 mRNA is actinomycin D-sensitive and is accompanied by a parallel (-3-fold) increase in secretion of TGF-81 protein in the culture medium of treated cells, as detected by immunoprecipitation of biosynthetically labeled S6S-labeled TGF-81 using specific anti-TGF-81 antibodies. Treatment of normal rat kidney cells with either epidermal growth factor or platelet-derived growth factor also results in an increase in TGF-81 mRNA (2-3-fold), although epidermal growth factor and TGF-81 appear to act via distinct mechanisms since their combined effects are greater than additive.