Selective cell affinity of biomimetic micro-nano-hybrid structured TiO2 overcomes the biological dilemma of osteoblasts
Selective cell affinity of biomimetic micro-nano-hybrid structured TiO2 overcomes the biological dilemma of osteoblasts
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DOI：
10.1016/j.dental.2009.11.077
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发表时间：
2010-04-01
期刊：
影响因子：
5
通讯作者：
Ogawa, Takahiro
中科院分区：
文献类型：
--
作者：
Hori, Norio;Iwasa, Fuminori;Ogawa, Takahiro
Objective.人们对牙科种植体表面有很大的需求，以加速种植体周围骨生成的过程，从而减少其愈合时间并实现早期加载。为此，成骨细胞的增殖和功能成熟（分化）之间的负相关性提出了快速生成大量骨的挑战。例如，成骨细胞在微粗糙的钛表面上表现出更快的分化但更慢的增殖。使用一个独特的微纳米级的TiO 2，模仿生物矿化基质的拓扑结构，这项研究表明，这一挑战可以克服，而不使用生物制剂。通过机械加工（光滑表面）制备2级商业纯钛的钛盘。为了产生具有峰和谷的微观纹理（微坑表面），对钛盘进行酸蚀刻。为了在微坑内产生200-nm的TiO 2纳米结（微坑中纳米结表面），TiO 2被沉积到酸蚀刻的表面上。将大鼠骨髓来源的成骨细胞和NIH 3 T3成纤维细胞培养在机械加工的光滑、微坑和微坑中纳米瘤表面上。尽管表面粗糙度显著增加，但在微坑中添加200 nm纳米结节增加了成骨细胞增殖，同时增强了其功能分化。相比之下，这种微凹中的纳米结节表面降低了成纤维细胞的增殖和功能。这些数据表明，建立细胞选择性功能化的纳米-微米智能钛表面，涉及成骨细胞增殖的调节作用，废除微坑表面上的抑制机制，同时增强其功能分化。仿生和可控性质的这种纳米结在微坑表面可以提供一种新的微米到纳米级的层次平台，以生物优化的生物材料的纳米功能。特别是，这种微纳米混合表面可能是一种有效的方法，以改善目前的牙种植体表面加速骨整合。(C)2009年牙科材料学院。由爱思唯尔有限公司出版。保留所有权利。
Objective. There is a great demand for dental implant surfaces to accelerate the process of peri-implant bone generation to reduce its healing time and enable early loading. To this end, an inverse correlation between the proliferation and functional maturation ( differentiation) in osteoblasts presents a challenge for the rapid generation of greater amounts of bone. For instance, osteoblasts exhibit faster differentiation but slower proliferation on micro-roughened titanium surfaces. Using a unique micro-nano-hierarchical topography of TiO2 that mimics biomineralized matrices, this study demonstrates that this challenge can be overcome without the use of biological agents.Methods. Titanium disks of grade 2 commercially pure titanium were prepared by machining ( smooth surface). To create a microtexture with peaks and valleys (micropit surface), titanium disks were acid-etched. To create 200-nm TiO2 nanonodules within the micropits (nanonodule-in-micropit surface), TiO2 was sputter-deposited onto the acid-etched surface. Rat bone marrow-derived osteoblasts and NIH3T3 fibroblasts were cultured on machined smooth, micropit, and nanonodule-in-micropit surfaces.Results. Despite the substantially increased surface roughness, the addition of 200-nm nanonodules to micropits increased osteoblast proliferation while enhancing their functional differentiation. In contrast, this nanonodule-in-micropit surface decreased proliferation and function in fibroblasts.Significance. The data suggest the establishment of cell-selectively functionalized nano-in-micro smart titanium surfaces that involve a regulatory effect on osteoblast proliferation, abrogating the inhibitory mechanism on the micropitted surface, while enhancing their functional differentiation. Biomimetic and controllable nature of this nanonodules-in-micropits surface may offer a novel micro-to-nanoscale hierarchical platform to biologically optimize nanofeatures of biomaterials. Particularly, this micro-nano-hybrid surface may be an effective approach to improve current dental implant surfaces for accelerated bone integration. (C) 2009 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.