Host Gender and Androgen Levels Regulate Gut Bacterial Taxa in Pigs Leading to Sex-Biased Serum Metabolite Profiles
Host Gender and Androgen Levels Regulate Gut Bacterial Taxa in Pigs Leading to Sex-Biased Serum Metabolite Profiles
复制标题
宿主性别和雄激素水平调节猪肠道细菌分类群，导致血清代谢物谱存在性别偏见
DOI：
10.3389/fmicb.2019.01359
复制
发表时间：
2019-06-18
影响因子：
5.2
通讯作者：
Huang, Lusheng
中科院分区：
文献类型：
--
作者：
He, Maozhang;Gao, Jun;Huang, Lusheng
肠道微生物群调节宿主的新陈代谢和免疫力。肠道微生物群的系统发育组成受到包括宿主性别在内的多种因素的影响。在这项研究中，性别对肠道微生物组成的影响及其随后对猪血清代谢产物的影响进行了评估。采用16 S rRNA基因测序法对293头纯种杜洛克猪（108头后备母猪和185头全公猪）和64头来自8个品种嵌合F6群体的经验证猪的粪便样品的细菌组成进行了测定。28头80日龄的F6公猪被阉割，以评估雄激素对肠道微生物组成的影响。采用超高效液相色谱-四极杆飞行时间质谱联用技术（UPLC-QTOF/MS）对45头公猪和26头后备母猪的非靶向血清代谢物特征进行了测定。该研究观察到宿主性别对肠道微生物组成的明显影响，并确定了许多性别偏见的细菌类群。这些包括韦荣氏球菌科、罗斯拜瑞氏菌属、牛肝菌属和埃希氏菌属，在公猪中的丰度较高，密螺旋体属和拟杆菌属在后备母猪中的丰度较高。阉割显著地将公猪的粪便微生物群组成向后备母猪的粪便微生物群组成转移。与肥胖和能量收获相关的肠道微生物组的预测功能途径在后备母猪中富集，并且与后备母猪富集的细菌正相关。与肽酶和碳水化合物代谢相关的功能途径在公猪中富集，并且与公猪富集的细菌正相关。与雄激素和甲酚代谢相关的血清代谢物被鉴定为性别偏倚代谢物。血清代谢产物与性别偏倚菌的相关性分析表明，血清雄激素相关代谢产物浓度与牛小泡菌属和埃希氏菌属呈正相关，与密螺旋体呈负相关，提示肠道菌群与宿主性激素代谢存在显著的相互作用。这些结果提供了宿主性别和肠道微生物群如何影响宿主代谢的基本知识。
Gut microbiota regulates host metabolism and immunity. The phylogenetic composition of gut microbiota is influenced by diverse factors that include host gender. In this study, the effects of gender on gut microbial composition and its subsequent influence on serum metabolites in pigs were evaluated. The bacterial composition of feces samples was determined by 16S rRNA gene sequencing in 293 pure-bred Duroc pigs (108 gilts and 185 entire boars) and 64 validated pigs from an eight-breed mosaic F6 population. Twenty-eight F6 boars were castrated at 80 days of age to evaluate the effects of androgen on gut microbial composition. Untargeted serum metabolite features were determined in 45 boars and 26 gilts by an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS). The study observed an obvious influence of host gender on the gut microbial composition and identified numerous sex-biased bacterial taxa. These included Veillonellaceae, Roseburia, Bulleidia, and Escherichia which showed the higher abundance in boars, and Treponema and Bacteroides which were over-represented in gilts. Castration significantly shifted the fecal microbiota composition of the boars toward that of gilts. The predicted functional pathways of the gut microbiome related to obesity and energy harvest were enriched in gilts, and positively associated with gilt-enriched bacteria. Functional pathways related to peptidases and carbohydrate metabolism were enriched in boars and positively associated with boar-enriched bacteria. Serum metabolites related to androgen and cresol metabolism were identified as sex-biased metabolites. Correlation analysis between serum metabolites and sex-biased bacteria identified that the serum concentration of androgen-related metabolites was positively correlated with Bulleidia and Escherichia, but negatively associated with Treponema, suggesting a significant interaction between gut microbiota and host sex hormone metabolism. These results offer basic knowledge of how host gender and gut microbiota influence host metabolism.