ORGANIZATION OF SEROTONIN-1A AND SEROTONIN-1B RECEPTORS IN THE NUCLEUS OF THE SOLITARY TRACT
ORGANIZATION OF SEROTONIN-1A AND SEROTONIN-1B RECEPTORS IN THE NUCLEUS OF THE SOLITARY TRACT
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DOI：
10.1002/cne.903010405
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发表时间：
1990-11-22
影响因子：
2.5
通讯作者：
VERDERAME, HM
中科院分区：
文献类型：
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作者：
MANAKER, S;VERDERAME, HM
用~ 3 H-8-羟基-N，N-二丙基-2-氨基四氢萘（~ 3 H-DPAT）和~（125）I-DPAT分别标记大鼠脑切片中的5-羟色胺（5 HT）1A和5 HT 1B受体，并对其药理学特性进行了研究。用定量放射自显影法测定迷走神经背侧复合体中5-HT 1A和5-HT 1B受体的浓度。NTS的中央和中间亚核内观察到最高的5 HT 1A受体浓度，与少量的3 H-DPAT结合位点观察到的舌下神经核和迷走神经背侧运动核。与此相反，5 HT 1B受体的密度是相对均匀的，通过所有NTS亚核的最高浓度位于腹外侧亚核。舌下神经和背侧运动核的5 HT 1B受体密度略高于NTS亚核，而最后区的密度非常低。这些数据表明，5 HT 1A受体的组织方式与细胞结构和hodological parcellation的NTS到个别亚核。中央和中间亚核中的5 HT 1A受体的高浓度表明这些受体在延髓反射途径subserving吞咽中的作用。在腹外侧亚核中相对高密度的5 HT 1B受体表明，这些受体调节呼吸神经元，而在其余亚核中的5 HT 1B受体的弥散组织表明，它们与中枢5 HT传入NTS的途径有关。需要进一步的研究来了解NTS内5 HT 1受体的生理作用。
We utilized 3H-8-hydroxy-N,N-dipropyl-2-aminotetralin (3H-DPAT) and 125-iodocyanopindolol (125I-CYP) to label serotonin (5HT) 1A and 5ht1B receptors, respectively, in sections of the rat brain after chracterizing the pharmacologic specificity of these agents. We then used quantitative autoradiography to measure the concentrations of 5HT1A and 5HT1B receptors in dorsal vagal complex. The highest 5HT1A receptor concentrations were observed within the central and intermediate subnuclei of the NTS, with low quantities of 3H-DPAT binding sites observed in the hypoglossal nucleus and dorsal motor nucelus of the vagus. In contrast, the density of 5HT1B receptors was relatively homogeneous through all NTS subnuclei with the highest concentrations localized within the ventrolateral subnucleus. The hypoglossal and dorsal motornuclei had slightly higher 5HT1B receptor densities than the NTS subnuclei, whereas the area postrema had a very low density. These data suggest that 5HT1A receptors are organized in a manner consistent with the cytoarchitectural and hodological parcellation of the NTS into individual subnuclei. The high concentrations of 5HT1A receptors in the central and intermediate subnuclei suggest a role for these receptors in medullary reflex pathways subserving deglutition. The relatively high density of 5HT1B receptors in the ventrolateral subnucleus suggests that these receptors modulate respiratory neurons, whereas the diffuse organization of 5HT1B receptors in the remaining subnuclei suggests that they are associated with central 5HT afferent pathways to the NTS. Further studies will be required to understand the physiologic role of 5HT1 receptors within the NTS.