Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospital
Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospital
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DOI：
10.7554/elife.53886
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发表时间：
2020-04-14
期刊：
影响因子：
7.7
通讯作者：
Van Tyne, Daria
中科院分区：
文献类型：
--
作者：
Evans, Daniel R.;Griffith, Marissa P.;Van Tyne, Daria
耐多药细菌对健康构成严重威胁，尤其是在医院。可移动遗传元件的水平基因转移(HGT)促进了抗生素耐药性、毒力和环境持久性基因在医院病原菌之间的传播。我们在18个月的时间里筛选了来自一家医院的2173株与医疗保健相关的感染细菌的基因组，并在属于不同属的细菌中确定了相同的核苷酸区域。为了进一步解析这些共享的序列，我们对分离株的子集进行了长阅读测序，并生成了高度连续的基因组。然后，我们跟踪了所有2173个基因组中10个不同质粒的出现，并发现了不依赖于细菌传播的质粒转移的证据。最后，我们确定了两个可能在个体患者体内转移的质粒，包括一个可能转移给第二个患者的质粒。这项工作扩大了我们对HGT在医疗保健环境中的理解，并可以为限制耐药病原体在医院传播的努力提供信息。
Multidrug-resistant bacteria pose a serious health threat, especially in hospitals. Horizontal gene transfer (HGT) of mobile genetic elements (MGEs) facilitates the spread of antibiotic resistance, virulence, and environmental persistence genes between nosocomial pathogens. We screened the genomes of 2173 bacterial isolates from healthcare-associated infections from a single hospital over 18 months, and identified identical nucleotide regions in bacteria belonging to distinct genera. To further resolve these shared sequences, we performed long-read sequencing on a subset of isolates and generated highly contiguous genomes. We then tracked the appearance of ten different plasmids in all 2173 genomes, and found evidence of plasmid transfer independent from bacterial transmission. Finally, we identified two instances of likely plasmid transfer within individual patients, including one plasmid that likely transferred to a second patient. This work expands our understanding of HGT in healthcare settings, and can inform efforts to limit the spread of drug-resistant pathogens in hospitals.