The preparation of testate amoebae (Protozoa: Rhizopoda) samples from peat
The preparation of testate amoebae (Protozoa: Rhizopoda) samples from peat
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DOI：
10.1177/095968369700700207
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发表时间：
1997-06
期刊：
影响因子：
--
通讯作者：
D. Hendon;D. Charman
中科院分区：
文献类型：
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作者：
D. Hendon;D. Charman
泥炭中有壳变形虫的分析被证明是一种有用的新的定量评价泥炭地水文变化的技术。准备实验进行了泥炭提取库姆里格莫斯，诺森伯兰郡，英格兰，以评估不同的程序对动物群的浓度和物种组成的影响。用不同的技术处理6个样品，每个样品重复5次，有壳变形虫与200个石松孢子一起计数。所有适用于花粉提取的准备程序可将测试记录的数量减少80%，并将分类群记录的数量减少60%。氢氧化钾消化并没有破坏测试，但损坏了他们，使识别困难，虽然它能够提取更多的测试。一种简单的水基制备方法，通过筛分除去粗碎屑和细碎屑，似乎可以得到最好的结果，并且是对早期技术的改进，可以从样品中除去更多的外来物质。花粉分析样品中的有壳变形虫计数必须极其谨慎，无论是绝对值还是相对值，都不能代表泥炭的动物区系含量。
The analysis of testate amoebae (Protozoa: Rhizopoda) in peat is proving to be a useful new quantitat ive technique for assessing hydrological change on peatlands. Preparation experiments were carried out on peat extracted from Coom Rigg Moss, Northumberland, England, in order to assess the impact of different procedures on the concentration and species composition of the faunas. Five replicates of each of six samples were treated by different techniques and the testate amoebae counted together with 200 Lycopodium spores. All preparation procedures suitable for pollen extraction reduce the number of tests recorded by up to 80% and the number of taxa recorded by 60%. KOH digestion did not destroy tests but damaged them, making identification difficult, although it enabled extraction of a higher number of tests. A simple water-based prep aration, with sieving to remove coarse and fine detritus, appears to give the best results and is an improvement on earlier techniques in removing more extraneous material from the samples. Testate amoebae counts from samples prepared for pollen analysis must be viewed with extreme caution and are unrepresentative of the faunal content of the peat in both absolute and relative terms.