Inducible production of human macrophage growth factor, CSF-1
Inducible production of human macrophage growth factor, CSF-1
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人类巨噬细胞生长因子 CSF-1 的诱导产生
DOI：
10.1182/blood.v68.3.633.bloodjournal683633
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发表时间：
1986
期刊：
影响因子：
20.3
通讯作者：
E. Kawasaki
中科院分区：
文献类型：
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作者：
P. Ralph;M. Warren;MT Lee;J. Csejtey;J. Weaver;H. Broxmeyer;D. Williams;E. Stanley;E. Kawasaki
使用特异性放射受体测定和小鼠骨髓培养物中巨噬细胞集落生长的标准筛选一组人细胞系的集落刺激因子-1（CSF-1）的产生。发现胰腺癌细胞系MIA PaCa和PANC分泌高水平的CSF-1。在骨髓增殖测定中，来自这两个细胞系的活性被CSF-1特异性中和抗血清阻断，证实了该巨噬细胞生长因子的主要含量。当转移到各种无血清培养基中时，MIA PaCA细胞停止分泌CSF-1。无血清生产可以重新启动佛波醇肉豆蔻乙酸酯（PMA）。从无血清MIA PaCa细胞中纯化的CSF-1刺激总的和非粘附的单核人骨髓细胞形成14天集落。大多数集落完全由大的、分散的巨噬细胞组成，巨噬细胞被非特异性酯酶强烈染色。尽管CSF-1和其他CSF刺激了相似数量的人14天集落，但与鼠骨髓祖细胞相比，人骨髓祖细胞的增殖需要更多的CSF- 1。使用人CSF-1寡核苷酸探针对来自诱导的MIA PaCa细胞的mRNA进行北方分析，揭示了范围为1.5至4.5个碱基（kb）的多种CSF-1 mRNA。未诱导的无血清培养物仅显示最大的mRNA种类，表明血清去除干扰了与蛋白质合成和/或分泌相关的CSF-1 mRNA加工。CSF-1产生的调节可能是造血和巨噬细胞功能中的重要生理过程。
A panel of human cell lines was screened for production of colony- stimulating factor-1 (CSF-1) using a specific radioreceptor assay and criterion of macrophage colony growth in mouse bone marrow culture. The pancreatic carcinoma lines MIA PaCa and PANC were found to secrete high levels of CSF-1. In a bone marrow proliferation assay, the activities from these two lines were blocked by a CSF-1 specific neutralizing antiserum, confirming the predominant content of this macrophage growth factor. MIA PaCA cells stopped secreting CSF-1 when transferred to various serum-free media. Serum-free production could be reinitiated by phorbol myristic acetate (PMA). Purified CSF-1 from serum-free MIA PaCa cells stimulated the formation of 14-day colonies from total and nonadherent mononuclear human bone marrow cells. Most of the colonies consisted exclusively of large, dispersed macrophages that were intensely stained for nonspecific esterase. Although similar numbers of human 14-day colonies were stimulated by CSF-1 and other CSFs, more CSF- 1 was required for the proliferation of human as compared with murine bone marrow progenitors. Northern analysis of mRNA from induced-MIA PaCa cells, using a human CSF-1 oligonucleotide probe, revealed multiple species of CSF-1 mRNA ranging from 1.5 to 4.5 kilobases (kb). Uninduced, serum-free cultures showed only the largest mRNA species, suggesting that serum removal interfered with CSF-1 mRNA processing related to synthesis and/or secretion of the protein. Regulation of the production of CSF-1 may be an important physiological process in hematopoiesis and macrophage functioning.
巨噬细胞集落刺激因子（M-CSF）增强小鼠巨噬细胞分泌氧还原产物的能力。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Wing,EJ;Ampel,NM;Waheed,A;Shadduck,RK
通讯作者：
Shadduck,RK