STABILITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ANTIBODIES IN WHOLE-BLOOD DRIED ON FILTER-PAPER AND STORED UNDER VARIOUS TROPICAL CONDITIONS IN KINSHASA, ZAIRE
STABILITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ANTIBODIES IN WHOLE-BLOOD DRIED ON FILTER-PAPER AND STORED UNDER VARIOUS TROPICAL CONDITIONS IN KINSHASA, ZAIRE
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DOI：
10.1128/jcm.30.5.1179-1182.1992
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发表时间：
1992-05-01
影响因子：
9.4
通讯作者：
QUINN, TC
中科院分区：
文献类型：
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作者：
BEHETS, F;KASHAMUKA, M;QUINN, TC
在20周的时间内，在模拟扎伊尔金沙萨恶劣的热带田间条件的各种储存环境下，对滤纸上的全血斑点用于检测人类免疫缺陷病毒1型（HIV-1）抗体进行了评价。在储存的前6周内，通过酶免疫测定和Western印迹（免疫印迹），高滴度和低滴度HIV-1阳性参考样本的所有重复样本均保持阳性，在所有储存条件下，HIV-1阴性样本的所有重复样本均保持阴性。然而，长达20周的湿热储存条件导致酶免疫测定光密度比值逐渐下降，这在HIV-1抗体滴度低的样品中尤其明显。在20周的储存期内，恶劣的热带操作条件并未导致任何重复假阳性结果。使用带有干燥剂的不透气袋储存滤纸上的血点，提高了HIV-1抗体检测随时间推移的稳定性，建议在恶劣的热带野外环境中储存滤纸上的全血点。
The use of whole-blood spots on filter paper for the detection of antibody to human immunodeficiency virus type 1 (HIV-1) was evaluated during a 20-week period under a variety of storage environments simulating the harsh tropical field conditions in Kinshasa, Zaire. During the first 6 weeks of storage, all replicates of high- and low-titer HIV-1-positive reference samples remained positive by enzyme immunoassay and Western blotting (immunoblotting), and all replicates of HIV-1-negative samples remained negative under all storage conditions. However, hot and humid storage conditions for up to 20 weeks caused a progressive decline in enzyme immunoassay optical density ratio values, which was particularly noticeable in samples with a low HIV-1 antibody titer. Harsh tropical operational conditions did not cause any repeatedly false-positive results during the 20-week storage period. The use of gas-impermeable bags with desiccant for the storage of blood spots on filter paper improved the stability of HIV-1 antibody detection over time and is recommended for the storage of whole-blood spots on filter paper in harsh tropical field settings.