Asbestos-induced lung inflammation and epithelial cell proliferation are altered in myeloperoxidase-null mice
Asbestos-induced lung inflammation and epithelial cell proliferation are altered in myeloperoxidase-null mice
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DOI：
10.1158/0008-5472.can-05-1751
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发表时间：
2005-11-01
期刊：
影响因子：
11.2
通讯作者：
Mossman, BT
中科院分区：
文献类型：
--
作者：
Haegens, A;van der Vliet, A;Mossman, BT
石棉纤维是导致氧化应激和炎症的致癌物质，但人们对石棉产生氧化剂的来源和后果知之甚少。在这里，我们发现吸入温石棉纤维会导致支气管肺泡灌洗液（BALF）中的髓过氧化物酶活性增加，以及远端细支气管和肺泡管内衬上皮细胞（石棉纤维最初沉积在肺中的部位）中的髓过氧化物酶免疫反应性增加。与假手术小鼠相比，石棉暴露的髓过氧化物酶缺失 (MPO-/-) 和正常 (MPO+/+) 小鼠在吸入石棉 9 天后，BALF 中的多形核白细胞（主要是中性粒细胞）出现了相当的增加。 BALF 上的差异细胞计数显示，在所有接触石棉的小鼠中，巨噬细胞的比例减少，淋巴细胞增加，但与正常小鼠相比，接触石棉的髓过氧化物酶缺失小鼠的巨噬细胞数量总体减少。髓过氧化物酶缺失小鼠中石棉相关的肺部炎症减少（P
Asbestos fibers are carcinogens causing oxidative stress and inflammation, but the sources and ramifications of oxidant production by asbestos are poorly understood. Here, we show that inhaled chrysotile asbestos fibers cause increased myeloperoxidase activity in bronchoalveolar lavage fluids (BALF) and myeloperoxidase immunoreactivity in epithelial cells lining distal bronchioles and alveolar ducts, sites of initial lung deposition of asbestos fibers. In comparison with sham mice, asbestos-exposed myeloperoxidase-null (MPO-/-) and normal (MPO+/+) mice exhibited comparable increases in polymorphonuclear leukocytes, predominately neutrophils, in BALF after 9 days of asbestos inhalation. Differential cell counts on BALF revealed decreased proportions of macrophages and increased lymphocytes in all mice exposed to asbestos, but numbers were decreased overall in asbestos-exposed myeloperoxidase-null versus normal mice. Asbestos-associated lung inflammation in myeloperoxidase-null mice was reduced (P