FATES OF THE BLASTOMERES OF THE 16-CELL STAGE XENOPUS EMBRYO
FATES OF THE BLASTOMERES OF THE 16-CELL STAGE XENOPUS EMBRYO
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DOI：
10.1016/0012-1606(87)90059-5
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发表时间：
1987-02-01
影响因子：
2.7
通讯作者：
MOODY, SA
中科院分区：
文献类型：
--
作者：
MOODY, SA
用辣根过氧化物酶在细胞内标记单个卵裂球，并用组织化学方法鉴定标记后的尾胚子代，确定了精心挑选的16细胞期非洲爪蟾胚胎中每个卵裂球的命运。每个卵裂球都有三个初级胚层。每个卵裂球的子代在表型和位置上都有其特有的分布。例如，大多数器官都是由特定卵裂球的后代组成的。此外，在一个器官内，单个卵裂球的后代被限制在确定的空间地址。本研究描述了鉴定的16细胞阶段卵裂球的命运，并证明如果胚胎被预先选择进行刻板分裂，它们是不同的和可预测的。
The fate of each of the blastomeres in the 16-cell stage Xenopus embryos which had been carefully selected for stereotypic cleavages was determined by intracellularly marking a single blastomere with horseradish peroxidase and identifying the labeled progeny in the tailbud embryo by histochemistry. Each blastomere populated all three primary germ layers. The progeny of each blastomere were distributed characteristically both in phenotype and in location. For example, most organs were populated by the descendants of particular sets of blastomeres. Furthermore, within an organ the progeny of a single blastomere were restricted to defined spatial addresses. This study describes the fates of identified 16-cell stage blastomeres and demonstrates that they are distinct and predictable if embryos are preselected for stereotypic cleavages.