The nitroxide Tempol affords protection against ultraviolet radiation in a transgenic murine fibroblast culture model of cutaneous photoaging
The nitroxide Tempol affords protection against ultraviolet radiation in a transgenic murine fibroblast culture model of cutaneous photoaging
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DOI：
10.1034/j.1600-0625.2001.100107.x
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发表时间：
2001-02-01
影响因子：
3.6
通讯作者：
Uitto, J
中科院分区：
文献类型：
--
作者：
Bernstein, EF;Kong, SK;Uitto, J
紫外线损伤皮肤的各种机制之一就是产生活性氧。Temol是一种超氧化物歧化酶类似物，当外源性给药时，它很容易穿透细胞膜，已被证明对某些形式的氧依赖损伤具有保护作用。在这项研究中，我们使用先前描述的转基因小鼠皮肤光老化模型，测量了Tempoll对紫外线A和紫外线B诱导的损伤的保护能力。体外检测Tempoll对紫外线辐射诱导的弹性蛋白启动子激活的抑制作用。在我们的体外系统中，坦波尔对紫外线B的保护作用超过50%，对紫外线A的保护作用超过70%。这些结果表明，超氧化物歧化酶模拟物Tempoll具有保护紫外线诱导的弹性蛋白启动子激活的能力。该化合物可能是一种有效的防止皮肤光老化的药理剂。
The generation of reactive oxygen species is among the various mechanisms by which ultraviolet radiation damages skin. Tempol, a superoxide dismutase analogue which readily penetrates cell membranes when administered exogenously, has been shown to provide protection against some forms of oxygen-dependent damage. In this study, we measured the ability of Tempol to protect against ultraviolet A- and ultraviolet B-induced damage, using a previously described transgenic mouse model of cutaneous photoaging. The ability of Tempol to prevent ultraviolet radiation-induced elastin promoter activation was determined in vitro. Tempol provided over 50% protection against ultraviolet B and over 70% protection against ultraviolet A as measured in our in vitro system. These results demonstrate the ability of the superoxide dismutase mimic, Tempol, to protect against ultraviolet induced elastin promoter activation. This compound could be a useful pharmacological agent to prevent cutaneous photoaging.