Construction and assessment of bio-engineered intervertebral discs.
Construction and assessment of bio-engineered intervertebral discs.
复制标题
生物工程椎间盘的构建和评估
DOI：
10.3892/etm.2017.4764
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发表时间：
2017-09
影响因子：
2.7
通讯作者：
Chen B
中科院分区：
文献类型：
--
作者：
Xiang H;Lin Y;Shen N;Wang Y;Wu X;Zhang G;Ma X;Chen B
本研究旨在探讨髓核细胞转化骨髓间充质干细胞（BM-MSCs）在椎间盘组织工程中的应用价值。将骨髓间充质干细胞和胎儿纳米粒进行体外培养，种植于聚乳酸-聚乙醇酸共聚物（PLGA）上，倒置和扫描电镜下观察。将具有粘附或悬浮的BM-MSC和NPs的PLGA支架植入新西兰白色兔的椎间盘中。12周后按Thompson分级评价椎体间信号强度。蛋白多糖和II型胶原蛋白分别进行了荧光定量和荧光化学测定。在与纳米粒共培养时，纺锤形或多角形BM-MSCs形成纤维样表型，并在附着于PLGA支架时以正常形态生长。PLGA支架组椎间蛋白聚糖表达和胶原II表达与植入BM-MSC和无支架NP的对照组相比存在显著差异（分别为3.93±0.31 vs. 3.52±0.26 mg/100 mg，12.70±2.83 vs. 9.50±2.06）。因此，BM-MSC可以与NP共培养以增强它们分化成用于椎间盘再生的NP。总之，PLGA支架提供了可行的生长条件，并允许维持工程化组织的机械性能和空间结构，这符合组织工程化椎间盘不退化的要求。
The present study assessed the value of bone marrow-mesenchymal stem cells (BM-MSCs) transformed by nucleus pulposus cells (NPs) for engineering of intervertebral discs. BM-MSCs and fetal NPs were cultured, planted onto polylactic acid-polyglycolic acid co-polymer (PLGA) and observed under inverted and scanning electron microscopes. PLGA scaffolds with adherent or suspended BM-MSCs and NPs were implanted into intervertebral discs of New Zealand white rabbits. Intervertebral signal intensity was evaluated by Thompson grading after 12 weeks. Proteoglycan and type II collagen were measured spectrophotometrically and immunohistochemically, respectively. Spindle or multi-angular BM-MSCs developed fibro-like phenotypesin co-culture with NPs and grew with a normal morphology when attached to PLGA scaffolds. A significant difference was observed in intervertebral proteoglycan expression and collagen II expression in the PLGA scaffold group vs. that in the control group implanted with BM-MSCs and NPs without a scaffold (3.93±0.31 vs. 3.52±0.26 mg/100 mg, 12.70±2.83 vs. 9.50±2.06, respectively). Thus, BM-MSCs can be co-cultured with NPs to enhance their differentiation into NPs for disc regeneration. In conclusion, PLGA scaffolds offer viable growing conditions and allow for the maintenance of mechanical properties and spatial structures of the engineered tissue, which meets the requirements of tissue-engineered discs that do not degenerate.
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影响因子：
--
作者：
McMeeken, J;Tully, E;Story, I
通讯作者：
Story, I
影响因子：
3
作者：
Sato, M;Asazuma, T;Fujikawa, K
通讯作者：
Fujikawa, K
影响因子：
3
作者：
THOMPSON, JP;PEARCE, RH;BISHOP, PB
通讯作者：
BISHOP, PB
DOI：
10.1016/j.berh.2010.10.002
发表时间：
2010-12-01
影响因子：
5.2
作者：
Hoy, D.;Brooks, P.;Buchbinder, R.
通讯作者：
Buchbinder, R.
影响因子：
6.2
作者：
Tse, WT;Pendleton, JD;Guinan, EC
通讯作者：
Guinan, EC