A Novel Genus of Actinobacterial Tectiviridae
A Novel Genus of Actinobacterial Tectiviridae
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DOI：
10.3390/v11121134
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发表时间：
2019-12-01
期刊：
影响因子：
4.7
通讯作者：
Erill, Ivan
中科院分区：
文献类型：
--
作者：
Caruso, Steven M.;deCarvalho, Tagide N.;Erill, Ivan
链霉菌StreptomycesEwheeHeim和Forthebois是Tectiviridae家族的两个新成员。这些细菌是在植物病原体疥螨链霉菌的培养物上分离的，该病原体以其对马铃薯作物的全球经济影响而闻名。透射电子显微镜显示病毒颗粒具有双层二十面体衣壳，并且经常出现具有领状结构的突出纳米管。质谱法证实了脂质的存在下，在病毒粒子，和一系列纯化的菌落从混浊的斑块和免疫试验表明，这两个细菌是温和的。链霉菌StreptomycesEschwheeHeim和Forthebois具有线性dsDNA染色体（分别为18，266 bp和18，251 bp长），具有特囊病毒科的特征性两段结构。两个基因组编码典型的tectiviral蛋白（主要衣壳蛋白，包装ATP酶和DNA聚合酶），以及PRD 1型病毒体相关的转糖基酶和膜DNA递送蛋白的同源物。比较基因组学和系统发育分析证实，这两种噬菌体与红球菌噬菌体Toil一起形成了Tectiviridae中的一个新属，我们暂时将其命名为Deltatectivirus。放线菌感染的tectiviruses与保守的基因组结构，但与其他tectiviruses属的成员很少的序列相似性的一个粘性分支的鉴定证实，Tectiviridae是一个古老的谱系感染广泛的细菌宿主。
Streptomyces phages WheeHeim and Forthebois are two novel members of the Tectiviridae family. These phages were isolated on cultures of the plant pathogen Streptomyces scabiei, known for its worldwide economic impact on potato crops. Transmission electron microscopy showed viral particles with double-layered icosahedral capsids, and frequent instances of protruding nanotubes harboring a collar-like structure. Mass-spectrometry confirmed the presence of lipids in the virion, and serial purification of colonies from turbid plaques and immunity testing revealed that both phages are temperate. Streptomyces phages WheeHeim and Forthebois have linear dsDNA chromosomes (18,266 bp and 18,251 bp long, respectively) with the characteristic two-segment architecture of the Tectiviridae. Both genomes encode homologs of the canonical tectiviral proteins (major capsid protein, packaging ATPase and DNA polymerase), as well as PRD1-type virion-associated transglycosylase and membrane DNA delivery proteins. Comparative genomics and phylogenetic analyses firmly establish that these two phages, together with Rhodococcus phage Toil, form a new genus within the Tectiviridae, which we have tentatively named Deltatectivirus. The identification of a cohesive clade of Actinobacteria-infecting tectiviruses with conserved genome structure but with scant sequence similarity to members of other tectiviral genera confirms that the Tectiviridae are an ancient lineage infecting a broad range of bacterial hosts.