The ribosomal RNA promoter of Acanthamoeba castellanii determined by transcription in a cell-free system.
The ribosomal RNA promoter of Acanthamoeba castellanii determined by transcription in a cell-free system.
复制标题
通过无细胞系统中的转录确定卡氏棘阿米巴的核糖体 RNA 启动子。
DOI：
10.1093/nar/13.17.6237
复制
发表时间：
1985
影响因子：
14.9
通讯作者：
Paule,MR
中科院分区：
文献类型：
--
作者：
Kownin,P;Iida,CT;Brown-Shimer,S;Paule,MR
确定了核糖体 RNA 转录忠实起始所需的 DNA 序列。 BAL-31 消化用于通过从 rDNA 模板的 3' 和 5' 末端引入缺失来修饰 rDNA 模板。利用来自卡氏棘阿米巴的体外转录系统单独测试所得突变DNA的模板活性或与野生型竞争。结果确定从 –31 延伸到 +8 的序列是转录所必需的。此外;当 –47 和 –32 之间的区域保持完整时，转录就会增强。
The DNA sequences required for faithful initiation of ribosomal RNA transcription were determined. BAL–31 digestion was used to modify the rDNA template by introducing deletions from its 3′–and 5′– ends. The resulting mutant DNAs were tested for template activity individually or in competition with wild type utilizing an in vitro transcription system from Acanthamoeba castellanii. The results identify the sequence extending from –31 to +8 to be absolutely required for transcription. In addition; when the region between –47 and –32 is left intact, transcription is augmented.