In vivo ablation of dendritic cell subset expressing the chemokine receptor XCR1.
In vivo ablation of dendritic cell subset expressing the chemokine receptor XCR1.
复制标题
表达趋化因子受体 XCR1 的树突状细胞亚群的体内消融。
DOI：
10.1007/978-1-4939-3606-9_17
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
T. Kaisho.
中科院分区：
文献类型：
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作者：
H. Hemmi;K. Hoshino;T. Kaisho.
树突状细胞(DC)是控制免疫应答的关键细胞群之一。为了建立体内的细胞耗竭系统，将人白喉毒素受体(DTR)在特定启动子的控制下转入表达DTR的小鼠体内。在这些小鼠中，DTR表达细胞在注射DT后被诱导耗尽。使用该系统对CD11c启动子下表达DTR的小鼠模型的分析，有助于我们通过耗尽CD11c+细胞来了解DC生物学。在DT处理后诱导消除特定DC亚群的其他小鼠模型也已经建立。在这里，我们描述了一种新的小鼠模型，在该模型中，XCR1+DC亚群在体内可诱导并短暂耗尽。
Dendritic cells (DCs) are one of the key populations controlling immune responses. To establish a cell depletion system in vivo, human diphtheria toxin (DT) receptor (DTR) is transduced to the mice in which DTR is expressed under the control of a specific promoter. In these mice, DTR-expressing cells are inducibly depleted after DT injection. Using this system, analysis of mouse models in which DTR was expressed under the CD11c promoter has contributed to our knowledge of DC biology by depleting CD11c+cells. Other mouse models to inducibly eliminate specific DC subsets upon DT treatment have been also generated. Here, we describe a new mouse model in which the XCR1+DC subset is inducibly and transiently depleted in vivo.