Cross-reactivity between the immunodominant determinant of the antigen I component of Streptococcus sobrinus SpaA protein and surface antigens from other members of the Streptococcus mutans group.
Cross-reactivity between the immunodominant determinant of the antigen I component of Streptococcus sobrinus SpaA protein and surface antigens from other members of the Streptococcus mutans group.
复制标题
远缘链球菌 SpaA 蛋白的抗原 I 成分的免疫显性决定簇与来自变形链球菌群其他成员的表面抗原之间的交叉反应性。
DOI：
10.1128/iai.58.7.2276-2282.1990
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发表时间：
1990
影响因子：
3.1
通讯作者：
Curtiss3rd,R
中科院分区：
文献类型：
--
作者：
Goldschmidt,RM;Curtiss3rd,R
变形链球菌微生物组的大多数成员指定一种主要的细胞表面相关蛋白SpaA，其由其抗原特性定义。来自远缘链球菌（Streptococcus sobrinus）6715的spaA基因的编码SpaA蛋白的主要抗原组分（抗原I）的免疫显性决定簇的区域最近已被表征。本研究检测了免疫显性决定簇的识别是否独立于免疫动物宿主，以及免疫显性决定簇引起的抗体是否与来自S.各种血清型的变异体。小鼠和兔抗血清的未变性的SpaA蛋白的反应类似的免疫显性决定簇和其他抗原结构的蛋白质在Western免疫印迹与SpaA多肽，指定的spaA基因片段在重组大肠杆菌中表达。这表明近交和远交动物的抗体应答相似。此外，针对S.重组E. coli中表达的蛋白质，以及从S. sobrinus对S.在免疫斑点和蛋白质印迹分析中，分别检测各种血清型的变形杆菌。这表明，对于S。sobrinus血清型g，针对SpaA蛋白的免疫应答由抗原I的免疫显性决定簇控制。此外，这表明含有免疫显性决定簇的SpaA蛋白结构域与赋予S.各种血清型的变异体。
Most members of the Streptococcus mutans group of microorganisms specify a major cell surface-associated protein, SpaA, that is defined by its antigenic properties. The region of the spaA gene from Streptococcus sobrinus 6715 encoding the immunodominant determinant of the major antigenic component (antigen I) of the SpaA protein has recently been characterized. This study examined whether recognition of the immunodominant determinant is independent of the immunized animal host and whether antibodies elicited by the immunodominant determinant cross-react with cell surface proteins from S. mutans of various serotypes. Mouse and rabbit antisera to the undenatured SpaA protein reacted similarly both with the immunodominant determinant and with other antigenic structures of the protein in Western immunoblots with SpaA polypeptides that were specified by spaA gene fragments expressed in recombinant Escherichia coli. This suggests that the antibody responses of inbred and outbred animals were similar. Furthermore, antibodies raised against both the S. sobrinus SpaA immunodominant determinant expressed by recombinant E. coli and the purified protein from S. sobrinus displayed similar strain specificities and protein band profiles towards cells surface proteins from S. mutans of various serotypes in immunodot and Western blot analyses, respectively. This suggests that for S. sobrinus serotype g, the immune response against the SpaA protein is governed by the immunodominant determinant of antigen I. In addition, it indicates that the SpaA protein domain containing the immunodominant determinant overlaps the domain conferring cross-reactivity to cell surface proteins of S. mutans of various serotypes.