Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa
Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa
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DOI：
10.1073/pnas.96.26.15202
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发表时间：
1999-12-21
影响因子：
11.1
通讯作者：
Manoil, C
中科院分区：
文献类型：
--
作者：
Darby, C;Cosma, CL;Manoil, C
识别与病原体相互作用的宿主因子对于理解传染病至关重要，但直接筛查宿主突变以帮助完成这一任务在哺乳动物中是不可行的。线虫秀丽隐杆线虫是一种遗传学上易于处理的替代研究病原菌铜绿假单胞菌。铜绿假单胞菌毒素，在群体感应调节因子LasR和RhlR的控制下，在高细胞密度下产生，迅速并致命地麻痹C。优美的C.功能丧失突变线虫egl-9是正常产卵所需的基因，它赋予线虫对麻痹的强大抵抗力。因此，EGL-9或包括EGL-9的通路的激活可能导致瘫痪。通过转化拯救和DNA测序确定egl-9的分子身份。EGL-9的哺乳动物同源物在暴露于铜绿假单胞菌可能具有临床效应的组织中表达。
Identification of host factors that interact with pathogens is crucial to an understanding of infectious disease, but direct screening for host mutations to aid in this task is not feasible in mammals. The nematode Caenorhabditis elegans is a genetically tractable alternative for investigating the pathogenic bacterium Pseudomonas aeruginosa. A P. aeruginosa toxin, produced at high cell density under control of the quorum-sensing regulators LasR and RhlR, rapidly and lethally paralyzes C. elegans. Loss-of-function mutations in C. elegans egl-9, a gene required for normal egg laying, confer strong resistance to the paralysis. Thus, activation of EGL-9 or of a pathway that includes it may lead to the paralysis. The molecular identity of egl-9 was determined by transformation rescue and DNA sequencing. A mammalian homologue of EGL-9 is expressed in tissues in which exposure to P. aeroginosa could have clinical effects.