RNA polymerase II is required for RNAi-dependent heterochromatin assembly
RNA polymerase II is required for RNAi-dependent heterochromatin assembly
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DOI：
10.1126/science.1114955
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发表时间：
2005-07-15
期刊：
影响因子：
56.9
通讯作者：
Murakami, Y
中科院分区：
文献类型：
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作者：
Kato, H;Goto, DB;Murakami, Y
在粟酒裂殖酵母中，RNA干扰（RNAi）机制将近着丝粒转录物转化为小干扰RNA（siRNA），并且是近着丝粒异染色质组装所必需的。在这里，我们描述A。RNA聚合酶II第二大亚基（RNAPII）突变。野生型和突变型RNAPII均定位于着丝粒周围。然而，突变导致异染色质组蛋白修饰的丢失和近着丝粒转录物的积累，伴随着siRNA的丢失。这种表型类似于RNAi中的突变体，表明RNAPII将近着丝粒转录与siRNA加工和异染色质组装偶联。
In Schizosaccharomyces pombe, the RNA interference (RNAi) machinery con. verts pericentromeric transcripts into small interfering RNAs (siRNAs) and is, required for the assembly of pericentromeric heterochromatin. Here we describe a. mutation in the second largest subunit of RNA polymerase II (RNAPII). Both wild-type and mutant RNAPII localized to the pericentromere. However, the mutation resulted in the loss of heterochromatic histone modifications and in the accumulation of pericentromeric transcripts, accompanied by the loss of siRNAs. This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly.