Responses of canola (Brassica napus L.) cultivars under contrasting temperature regimes during early seedling growth stage as revealed by multiple physiological criteria
Responses of canola (Brassica napus L.) cultivars under contrasting temperature regimes during early seedling growth stage as revealed by multiple physiological criteria
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DOI：
10.1007/s11738-014-1748-9
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发表时间：
2015-01
影响因子：
2.6
通讯作者：
Jing Zhang;F. Jiang;Ping Yang;Jing Li;G. Yan;Liyong Hu
中科院分区：
文献类型：
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作者：
Jing Zhang;F. Jiang;Ping Yang;Jing Li;G. Yan;Liyong Hu
以油菜苗期最适温度(25/20)为对照，研究了甘蓝型油菜在35/30(高温)和15/10(低温)两种不同昼夜温度条件下的反应。结果表明，高温抑制成苗，低温抑制种子萌发。高温和低温均对苗木生长产生不利影响，表现为苗木的成苗率、苗木活力指数和鲜重均因积累反应氧而降低。抗氧化物酶对高温和低温的反应不同。高温下，超氧化物歧化酶(SOD)和过氧化物酶(POD)活性下降，过氧化氢酶(CAT)活性上升。而在低温下，SOD活性升高，POD活性降低，CAT活性不变。脯氨酸在油菜幼苗对温度胁迫的耐性中起着重要作用，可作为油菜幼苗对不利温度条件的耐性指标。与供试的其他品种相比，华优杂9号表现出更强的耐高低温能力。全面的耐受机制还需要进一步研究。
Investigations were carried out to study the responses of canola (Brassica napus) under contrasting temperature regimes (day/night °C): 35/30 (high temperature) and 15/10 (low temperature) in comparison with optimal temperature (25/20) at early seedling stage. The results indicated that high temperature inhibited seedling establishment, while low temperature restrained seed germination. Both high and low temperatures showed detrimental effects on seedling growth as revealed by reduced establishment percentage, seedling vigor index, and fresh weight due to accumulated reactive oxygens. The antioxidant enzymes responded to high and low temperature differently. Under high temperature, superoxide dismutase (SOD) and peroxidase (POD) activities reduced, while catalase (CAT) activities increased. Under low temperature, however, SOD activities increased, while POD activities reduced, with CAT activities unchanged. Proline played an important role in temperature stress tolerance and can be used as an indicator for tolerance to unfavorable temperature conditions in canola seedlings. Huayouza 9 showed much stronger tolerance to both high and low temperature compared with other cultivars tested in this study. The full extent of tolerance mechanisms need to be further studied.