MORPHOMETRIC QUANTITATION OF STROMA IN HUMAN BENIGN PROSTATIC HYPERPLASIA
MORPHOMETRIC QUANTITATION OF STROMA IN HUMAN BENIGN PROSTATIC HYPERPLASIA
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DOI：
10.1016/s0090-4295(94)80011-1
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发表时间：
1994-07-01
期刊：
影响因子：
2.1
通讯作者：
BRAWER, MK
中科院分区：
文献类型：
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作者：
DEERING, RE;BIGLER, SA;BRAWER, MK
目标。人类良性前列腺增生（BPH）由三个主要的组织学组成：间质、上皮和腔隙。为了减轻由前列腺增生引起的膀胱出口梗阻，量化前列腺增生的组织学组成可能有助于选择治疗方法。为了研究BPH间质百分比在患者之间的差异，我们对临床上明显的膀胱出口梗阻患者的标本进行了定量形态学测量，这些标本通过三种手术获得：开放性前列腺切除术、经尿道前列腺切除术（TURP）和前列腺穿刺活检（PNB）。方法。对每一种手术方式获得的10个标本进行分析。用肌动蛋白抗体对标本进行染色，标记基质；通过计算机图像分析完成基质的定量。结果。间质在所有BPH病例中的比例为49.9% ~ 76.7%（平均65.4%,SD = 7.4）。在比较三种方法获得的样本时，没有观察到显著差异（p = 0.70）。PNB标本的平滑肌间质成分也被量化。对于这些样本，活检中基质的百分比与由平滑肌组成的基质的百分比呈显著的负相关（r2 = 0.49; p = 0.021）。结论。数据表明，BPH的最大组成部分是间质，约占增生组织总数的50%至75%。无论是开放性前列腺切除术的大组织样本还是针刺活检的小样本，基质百分比的平均值和范围都是相似的。PNB数据表明，间质百分比的增加可能是由于间质中非肌肉成分的增加。
Objectives. Human benign prostatic hyperplasia (BPH) consists of three major histologic components: stroma, epithelium, and luminal space. For the relief of bladder outlet obstruction caused by BPH, quantitation of the histologic composition of BPH may aid in selecting treatment. To investigate variation between patients in the stromal percentage of BPH, we performed quantitative morphometry on specimens from patients with clinically significant bladder outlet obstruction obtained by three procedures: open prostatectomy, transurethral resection of the prostate (TURP), and prostate needle biopsy (PNB) just prior to TURP. Methods. Ten specimens obtained by each surgical procedure were analyzed. Specimens were stained with antibody to muscle-specific actin to mark the stroma; quantitation of stroma was accomplished by computer image analysis. Results. The percentage of stroma in all BPH cases ranged from 49.9% to 76.7% (mean, 65.4%, SD = 7.4). No significant difference was observed when comparing samples obtained by the three procedures (p = 0.70). Smooth muscle stromal composition was also quantified in PNB specimens. For these samples, a significant inverse relationship was found between the percentage stroma in the biopsy and the percentage of stroma composed of smooth muscle (r2 = 0.49; p = 0.021). Conclusions. The data demonstrated that the largest component of BPH was stroma, which comprised approximately 50% to 75% of the total hyperplastic tissue. The mean and range of stromal percentage were similar whether investigating large tissue samples from open prostatectomies or small samples from needle biopsies. PNB data indicated that an increased percentage of stroma may be due to increased nonmuscular elements in the stroma.