A ribosomal protein L2 gene is transcribed, spliced, and edited at one site in rice mitochondria
A ribosomal protein L2 gene is transcribed, spliced, and edited at one site in rice mitochondria
复制标题
核糖体蛋白 L2 基因在水稻线粒体的一个位点进行转录、剪接和编辑
作者：
N. Kubo;K. Ozawa;T. Hino;K. Kadowaki
水稻线粒体核糖体蛋白L2基因（rpl2）由840和669 bp的外显子和1481 bp的内含子组成。rpl2基因位于核糖体蛋白S19基因（rps19）上游三个核苷酸，并且两个基因共转录。cDNA序列分析鉴定了来自rpl2 mRNA的内含子序列的剪接以及RNA编辑事件。推导的rpl2内含子序列的二级结构显示了II组内含子的特征。在rpl2中鉴定了单个RNA编辑位点，在rps19转录物中鉴定了六个编辑位点。此外，在3个核苷酸的基因间区域中观察到一个编辑位点。对单个cDNA克隆的分析显示了不同程度的RNA编辑。水稻rpl2内含子位于不同的位点，与苔类植物的rpl2内含子没有显着的核苷酸序列相似性。然而，在水稻rpl2内含子和月见草nad5内含子之间的234个核苷酸区域中观察到60%的核苷酸序列同一性。豌豆线粒体基因组中不存在线粒体RPL2序列，因此我们提出线粒体RPL2蛋白由豌豆中的核基因编码。
The mitochondrial ribosomal protein L2 gene (rpl2) is coded by two exons of 840 and 669 bp separated by an intron sequence of 1481 bp in the rice mitochondrial genome. The rpl2 gene is located three nucleotides upstream of the ribosomal protein S19 gene (rps19) and both genes are co-transcribed. cDNA sequence analysis indentified splicing of the intron sequence from the rpl2 mRNA as well as RNA editing events. The deduced secondary structure of the rpl2 intron sequence shows the characteristic features of a group-II intron. A single RNA editing site is identified in rpl2 and six editing sites in rps19 transcripts. In addition, one editing site is observed in the 3 nucleotide intergenic region. Analysis of individual cDNA clones showed a different extent of RNA editing. The rice rpl2 intron is located at a different site and shows no significant nucleotide sequence similarity with the rpl2 intron of liverwort. However, 60% nucleotide sequence identity is observed between the rice rpl2 intron and the Oenothera nad5 intron in a 234 nucleotide region. The mitochondrial rpl2 sequence is absent from the pea mitochondrial genome and we consequently propose that the mitochondrial RPL2 protein is encoded by a nuclear gene in pea.
影响因子：
11.4
作者：
HUNT, MD;NEWTON, KJ
通讯作者：
NEWTON, KJ