Direct visualization of glucocorticoid receptor positive cells in the hippocampal regions using green fluorescent protein transgenic mice
Direct visualization of glucocorticoid receptor positive cells in the hippocampal regions using green fluorescent protein transgenic mice
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DOI：
10.1016/j.neuroscience.2007.03.020
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发表时间：
2007-06-08
期刊：
影响因子：
3.3
通讯作者：
Kawata, M.
中科院分区：
文献类型：
--
作者：
Nishi, M.;Usuku, T.;Kawata, M.
海马体是一种可塑性的大脑结构，对某些类型的学习和记忆很重要，也容易受到压力和创伤的影响。由于海马神经元表达高水平的皮质类固醇受体，因此应激释放的糖皮质激素引起的形态变化，包括胞体大小的改变，以及神经突和棘的长度和数量的变化，令人感兴趣。为了突出表达糖皮质激素受体（GR）的神经元形态，我们在GR启动子的控制下，构建了表达绿色荧光蛋白（GFP）的转基因小鼠品系。我们发现转基因小鼠脑切片CA1和CA2区锥体细胞层和海马齿状回颗粒细胞层有较强的绿色荧光。GFP荧光不仅在胞体中可见，在包括树突和轴突在内的神经突中也可见。在游离培养中，我们还在体细胞、包括树突和轴突在内的神经突以及树突棘中观察到GFP荧光。微管相关蛋白2免疫阳性锥体形神经元明显显示出两种不同的群体，GFP阳性和GFP阴性神经元。这些结果表明，该转基因小鼠系可用于动物神经元结构的实时成像以及以GFP作为特异性指示物的gr阳性培养细胞。(c) 2007 ibro。Elsevier Ltd.出版。版权所有。
The hippocampal formation is a plastic brain structure important for certain types of learning and memory, and also vulnerable to the effects of stress and trauma. Since hippocampal neurons express high levels of corticosteroid receptor, the morphological changes, including alterations in the size of soma, and the length and number of neurites and spines, in response to glucocorticoids released as a result of stress are intriguing. In order to highlight the morphology of neurons that express glucocorticoid receptor (GR), we have generated a transgenic mouse line expressing green fluorescent protein (GFP) under the control of the GR promoter. We found strong green fluorescence in the pyramidal cell layer of the CA1 and CA2 regions and the granule cell layer of the dentate gyrus of the hippocampus in brain sections of the transgenic mice. GFP fluorescence was observed not only in somas, but also in neurites including both dendrites and axons. In dissociated culture, we also observed GFP fluorescence in the soma, neurites including both dendrites and axons, and dendritic spines. Microtubule-associated protein 2 immunopositive pyramidal-shaped neurons clearly showed two different populations, GFP positive and GFP negative neurons. These results indicate that this transgenic mouse line should be useful for live imaging of neuronal structure in animals as well as GR-positive cultured cells using GFP as a specific indicator. (C) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.