The rabies virus glycoprotein determines the distribution of different rabies virus strains in the brain
The rabies virus glycoprotein determines the distribution of different rabies virus strains in the brain
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DOI：
10.1080/13550280290100707
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发表时间：
2002-08-01
影响因子：
3.2
通讯作者：
Fu, ZF
中科院分区：
文献类型：
--
作者：
Yan, XZ;Mohankumar, PS;Fu, ZF
用免疫组织化学方法观察了狂犬病病毒（RV）糖蛋白（G）在脑内分布中的作用。本研究中使用的病毒包括高度神经侵袭性攻毒病毒标准株（CVS-N2 C和CVS-B2C）和非神经侵袭性减毒株SN-10，以及表达CVS-N2 C（RN 2C）或CVS-B2C（RB 2C）G基因的SN-10衍生重组病毒。重组病毒在脑中的分布与G所来源的亲本病毒的分布相似。例如，CVS-B2C和RB 2C感染的神经元优先出现在海马、皮质和下丘脑中，而CVS-N2 C和RN 2C感染的神经元优先出现在海马、皮质和丘脑中。SN-10有效地感染了几乎所有的大脑区域。为了进一步研究RV G在病毒传播中的作用，我们检测了用重组RV感染的脑中RV抗原的分布，其中SN-10 G被水泡性口炎病毒（VSV）G（SN-10-VG）取代。SN-10-VG向皮质和丘脑的扩散急剧减少，但海马和下丘脑，特别是室旁核中受感染的神经元数量与SN-10病毒相似。这种传播模式类似于VSV。总之，我们的数据表明，这是G蛋白决定RV在大脑中的分布模式。
The contribution of rabies virus (RV) glycoprotein (G) in viral distribution in the brain was examined by immunohistochemistry following stereotaxic inoculation into the rat hippocampus. Viruses used in this study include the highly neuroinvasive challenge virus standard strains (CVS-N2C and CVS-B2C) and the nonneuroinvasive attenuated SN-10 strain, as well as SN-10-derived recombinant viruses expressing the G gene from CVS-N2C (RN2C) or CVS-B2C (RB2C). The distribution of recombinant viruses in the brain was similar to those of the parental viruses from which the G was derived. For example, while CVS-B2C- and RB2C-infected neurons were seen preferentially in the hippocampus, cortex, and hypothalamus, CVS-N2C- and RN2C-infected neurons were preferentially found in the hippocampus, cortex, and thalamus. SN-10 infected efficiently almost all the brain regions. To further study the role of the RV G in virus spreading, we examined the distribution of RV antigen in brains infected with a recombinant RV in which the SN-10 G was replaced with vesicular stomatitis virus (VSV) G (SN-10-VG) was examined. The spreading of SN-10-VG to the cortex and the thalamus was drastically reduced, but the number of infected neurons in hippocampus and hypothalamus, particularly the paraventricular nucleus, was similar to the SN-10 virus. This pattern of spreading resembles that of VSV. Together, our data demonstrate that it is the G protein that determines the distribution pattern of RV in the brain.