Nutritional supplementation with antioxidants decreases chromosomal damage in humans
Nutritional supplementation with antioxidants decreases chromosomal damage in humans
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DOI：
10.1093/mutage/geg002
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发表时间：
2003-07-01
期刊：
影响因子：
2.7
通讯作者：
Collins, AR
中科院分区：
文献类型：
--
作者：
Dusinská, M;Kazimírová, A;Collins, AR
为了研究抗氧化剂补充对染色体损伤的影响，对28例心肌梗死幸存者和57例农村对照组进行了3个月的抗氧化剂补充试验，均为男性。补充剂包括维生素C(每天100毫克)、维生素E(每天100毫克)、β-胡萝卜素(每天6毫克)和硒(每天50毫克)。测定血浆中的膳食抗氧化剂，以及血浆铁还原能力(衡量血浆总抗氧化状态的指标)和作为氧化应激指标的丙二醛浓度。在补充期开始和结束时收集的淋巴细胞被刺激增殖，并积累中期，以计分染色体畸变率：异常细胞百分比、染色单体和染色体断裂。在农村对照组中，补充抗氧化剂与染色体畸变率下降有关(补充前为0.63%，补充后为0.27%；P=0.03)。在这组吸烟者中，补充的效果最大(补充0.12%的异常细胞，而安慰剂组为0.81%；P<；0.001)。这一结果支持了抗氧化剂可以减少遗传损伤的假设。
In order to investigate the effects of antioxidant supplementation on chromosome damage, a 3 month antioxidant supplementation trial was conducted on groups of 28 myocardial infarction survivors and 57 rural controls, all male. The supplement consisted of vitamin C (100 mg/day), vitamin E (100 mg/day), beta-carotene (6 mg/day) and selenium (50 mumg/day). Dietary antioxidants in plasma were measured, as well as the ferric reducing ability of plasma (a measure of total plasma antioxidant status) and the concentration of malondialdehyde as an indicator of oxidative stress. Lymphocytes collected at the beginning and end of the supplementation period were stimulated to proliferate and metaphases accumulated for scoring of chromosome aberrations: per cent aberrant cells and chromatid and chromosome breaks. Supplementation with antioxidants was associated with a decrease in the percentage of cells with chromosome aberrations in the group of rural controls (0.63% before compared with 0.27% after supplementation; P = 0.03). The largest effect of supplementation was seen in smokers in this group (0.12% aberrant cells in supplemented compared with 0.81% in placebo group; P < 0.001). The results support the hypothesis that antioxidants decrease genetic damage.