Lack of cytomegalovirus detection in human glioma.
Lack of cytomegalovirus detection in human glioma.
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DOI：
10.1186/s12985-017-0885-3
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发表时间：
2017-11-07
期刊：
影响因子：
4.8
通讯作者：
Barbera VM
中科院分区：
文献类型：
--
作者：
Garcia-Martinez A;Alenda C;Irles E;Ochoa E;Quintanar T;Rodriguez-Lescure A;Soto JL;Barbera VM
神经胶质瘤是最常见的脑肿瘤，包括各种组织学类型和恶性程度。它们起源于神经胶质细胞，占原发性脑肿瘤的约70%。根据世界卫生组织（WHO）的标准，大多数胶质瘤可分为四个恶性等级（I-IV）。病毒感染，特别是DNA病毒和逆转录病毒感染，可能导致病毒DNA序列插入宿主基因组，通常会触发宿主的防御机制。特别地，DNA甲基化机制可以被激活以引起外来可移动病毒序列的甲基化，并因此沉默病毒基因表达。几项研究表明，人巨细胞病毒（HCMV）在胶质母细胞瘤中的存在，表明该病毒可能参与肿瘤的发病机制。但这种关系是有争议的，因为许多其他研究没有在这些肿瘤中检测到HCMV。本研究旨在通过不同的敏感技术检测几种人脑胶质瘤标本（94例福尔马林固定石蜡包埋标本和28例速冻标本）中HCMV的存在。我们无法在胶质瘤样本中检测到HCMV DNA和蛋白。因此，我们认为，到目前为止得出结论认为HCMV是胶质瘤中的肿瘤调节病毒的论点必须认真重新考虑。本文的在线版本（10.1186/s12985-017-0885-3）包含补充材料，可供授权用户使用。
Gliomas are the most common brain tumors and include a variety of histologic types and grades of malignancy. They arise from glial cells and represent approximately 70% of the primary brain tumors. According to the criteria of the World Health Organization (WHO), the majority of gliomas can be classified into four grades of malignancy (I-IV). Virus infection, especially by DNA viruses and retroviruses, which may cause insertion of viral DNA sequences into the host genome, often triggers the host defense mechanisms. Particularly, the DNA methylation machinery can be activated to cause the methylation of foreign movable viral sequences and, therefore, silence viral gene expression. Several studies have shown the presence of Human Cytomegalovirus (HCMV) in glioblastoma, suggesting that the virus may participate in tumor pathogenesis. But this relationship is controversial because many other studies did not detect HCMV in these tumors. This study aims to detect the presence of HCMV in several samples of human glioma (94 formalin-fixed, paraffin-embedded samples and 28 snap-frozen samples) by different sensitive techniques. We have been unable to detect HCMV DNA and proteins in glioma samples. Therefore, arguments used so far to conclude that HCMV is an oncomodulator virus in gliomas must be, in our view, seriously reconsidered. The online version of this article (10.1186/s12985-017-0885-3) contains supplementary material, which is available to authorized users.
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DOI：
10.1158/1078-0432.ccr-13-1121
发表时间：
2013-12-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
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作者：
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期刊：
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影响因子：
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DOI：
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影响因子：
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作者：
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影响因子：
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