Ion irradiation in liquid of μm3 region for cell surgery
Ion irradiation in liquid of μm3 region for cell surgery
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DOI：
10.1063/1.2834695
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发表时间：
2008-01
影响因子：
4
通讯作者：
Y. Iwai;T. Ikeda;T. Kojima;Y. Yamazaki;K. Maeshima;N. Imamoto;Tomohiro Kobayashi;T. Nebiki;T. Narusawa;G. Pokhil
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文献类型：
--
作者：
Y. Iwai;T. Ikeda;T. Kojima;Y. Yamazaki;K. Maeshima;N. Imamoto;Tomohiro Kobayashi;T. Nebiki;T. Narusawa;G. Pokhil
我们在这里提出了一个细胞手术方案，涉及选择性失活或破坏细胞结构。高能离子通过锥形玻璃毛细管注入细胞，类似于显微注射方法。与显微注射的一个微小但本质的区别是，在出口处准备了一个薄窗口，使得没有液体材料可以通过出口流入或回流，同时仍然允许高能离子渗透到细胞中。从这样一个出口直径为10μm的毛细管中射出的10MeV He离子束灭活了位于HeLa细胞核中的选定体积（100 μm3）的荧光分子。
We present here a cell surgery scheme involving selective inactivation or disruption of cellular structures. Energetic ions are injected into a cell through a tapered glass capillary like a microinjection method. A slight but essential difference from microinjection is that a thin window is prepared at the outlet so that no liquid material can flow in or back through the outlet while still allowing energetic ions to penetrate into the cell. An ∼MeV He ion beam from such a capillary having 10μm outlet diameter inactivated a selected volume (∼μm3) of fluorescent molecules located in a HeLa cell nucleus.