CMRF-35-like molecule-5 constitutes novel paired receptors, with CMRF-35-like molecule-1, to transduce activation signal upon association with FcRγ
CMRF-35-like molecule-5 constitutes novel paired receptors, with CMRF-35-like molecule-1, to transduce activation signal upon association with FcRγ
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DOI：
10.1093/intimm/dxl083
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发表时间：
2006-10-01
影响因子：
4.4
通讯作者：
Ohno, Hiroshi
中科院分区：
文献类型：
--
作者：
Fujimoto, Masanori;Takatsu, Hiroyuki;Ohno, Hiroshi
小鼠CMRF-35样分子（CLM）家族属于IG超家族，由9个定位基因组成。在这里，我们报告，CLM-5和CLM-1构成新的成对的Ig样受体（PIR）。CLM-1和CLM-5基因在11号染色体上紧邻编码。据报道，CLM-1在其胞质区域具有基于免疫受体酪氨酸的抑制基序，并抑制破骨细胞的分化。CLM-5具有与CLM-1在其胞外结构域中高度同源的IG结构域，并且在其跨膜结构域中具有带负电荷的残基。与CLM-1一样，CLM-5在骨髓细胞中表达，例如树突细胞、巨噬细胞和粒细胞。我们还表明，CLM-5与FcR γ相互作用，在质膜上表达，CLM-5的交联导致蛋白质的酪氨酸磷酸化，包括FcR γ，在单核细胞/巨噬细胞系。综上所述，这些特征表明CLM-1和CLM-5构成了新的PIR，并且CLM-5可能作为髓样细胞中的活化受体传递活化信号。
The murine CMRF-35-like molecule (CLM) family belongs to the Ig superfamily, and consists of nine mapped genes. Here we report that CLM-5 and CLM-1 constitute novel paired Ig-like receptors (PIRs). CLM-1 and CLM-5 genes are encoded in close vicinity on chromosome 11. CLM-1 has been reported to possess immunoreceptor tyrosine-based inhibitory motifs in its cytoplasmic region and to inhibit the differentiation of osteoclast. CLM-5 has an Ig domain that is highly homologous with that of CLM-1 in its extracellular domain, and possesses a negatively charged residue in its transmembrane domain. CLM-5, like CLM-1, is expressed in myeloid cells, such as dendritic cells, macrophages and granulocytes. We also show that CLM-5 interacts with FcR gamma to be expressed on the plasma membrane and that the cross-linking of CLM-5 leads to tyrosine phosphorylation of proteins, including FcR gamma, in the monocyte/macrophage cell line. Taken together, these characteristics suggest that CLM-1 and CLM-5 constitute novel PIRs and that CLM-5 may transduce activation signals as the activating receptor in myeloid cells.