Collagen matrix promotes reorganization of pancreatic endocrine cell monolayers into islet-like organoids.
Collagen matrix promotes reorganization of pancreatic endocrine cell monolayers into islet-like organoids.
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DOI：
10.1083/jcb.97.3.935
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发表时间：
1983-09
期刊：
影响因子：
--
通讯作者：
Orci L
中科院分区：
文献类型：
--
作者：
Montesano R;Mouron P;Amherdt M;Orci L
为了根据在原位朗格汉斯胰岛中观察到的特征性地形模式评估胰腺内分泌细胞在体外重新结合的能力，我们在三维胶原基质内培养从新生大鼠胰腺分离的细胞。在胶原凝胶表面生长的细胞单层被第二层胶原覆盖。这诱导单层内分泌细胞重组成轮廓光滑的三维聚集体，其中非 B 细胞（通过电子显微镜和免疫荧光鉴定）优先分布在外围，而 B 细胞集中在中心位置。这些结果表明，培养的胰腺内分泌细胞具有在体外重新结合成胰岛样器官的能力，并且胶原蛋白基质可能对这种潜力的表达具有许可作用。
To evaluate the capacity of pancreatic endocrine cells to reassociate in vitro according to the characteristic topographical pattern observed in the islets of Langerhans in situ, we cultured cells dissociated from neonatal rat pancreas within a three-dimensional collagen matrix. Cell monolayers grown on the surface of collagen gels were covered with a second layer of collagen. This induced the monolayers of endocrine cells to reorganize into smooth-contoured, three-dimensional aggregates, in which non-B cells (identified by electron microscopy and immunofluorescence) had a preferential distribution at the periphery, whereas B cells were concentrated in a central position. These results show that cultured pancreatic endocrine cells have the capacity to reassociate into islet-like organoids in vitro, and that collagen matrices may have a permissive effect on the expression of this potential.