Enhancing the effects of exfoliated carbon nanofibers using bone morphogenetic protein in a rat spinal fusion model
Enhancing the effects of exfoliated carbon nanofibers using bone morphogenetic protein in a rat spinal fusion model
复制标题
在大鼠脊柱融合模型中使用骨形态发生蛋白增强脱落碳纳米纤维的效果
DOI：
10.1002/jor.24073
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Tsumura Hiroshi
中科院分区：
文献类型：
--
作者：
Notani Naoki;Miyazaki Masashi;Toyoda Masahiro;Kanezaki Shozo;Ishihara Toshinobu;Tsumura Hiroshi
剥离碳纳米纤维(ExCNF)有望成为促进和引导骨组织再生的良好支架材料。我们的目的是利用骨形态发生蛋白(BMPs)增强ExCNFs的效果，并使用大鼠脊柱融合模型来检验它们在临床应用中的可行性和安全性。I组(n= 15)植入对照载体，II组(n= 16)植入1 μg ExCNF载体，III组(n= 16)植入1 μg重组人(Rh)骨形态发生蛋白-2载体，IV组(n= 17)植入1 μg hBMP-2和1 μg ExCNF载体。于术后4周或8周处死大鼠，取其脊柱进行人工触诊、X线片和高分辨率微电脑断层扫描(Micro-CT)检查，并进行组织学分析。IV组融合率(25.0%：4周，45.5%：8周)明显高于I组(0%：4周，0%：8周)、II组(0%：4周，15.0%：8周)和III组(16.7%：4周，30.0%：8周)。这些结果证实了BMP在大鼠脊柱融合模型中对ExCNF骨融合的促进作用。我们的结果提示，骨形态发生蛋白对ExCNFs效应的增强可能使其成为脊柱融合手术的一种有吸引力的治疗方法。©2018矫形外科研究会。由Wiley期刊出版公司J Orthop Res 36：2892-2900,2018。
Exfoliated carbon nanofibers (ExCNFs) are expected to serve as excellent scaffolds for promoting and guiding bone‐tissue regeneration. We aimed to enhance the effects of ExCNFs using bone morphogenetic proteins (BMPs) and examined their feasibility and safety in clinical applications using a rat spinal fusion model. Group I (n= 15) animals were implanted with the control carrier; Group II (n= 16) animals were implanted with carrier containing 1 μg ExCNFs; Group III (n= 16) animals were implanted with carrier containing 1 μg recombinant human (rh) BMP‐2; and Group IV (n= 17) animals were implanted with carrier containing 1 μg rhBMP‐2 and 1 μg ExCNFs. The rats were euthanized after 4 or 8 weeks and their spines were explanted and assessed by manual palpation, radiographs, and high‐resolution microcomputerized tomography (micro‐CT); the spines were also subjected to histological analysis. The fusion rates in Group IV (25.0%: 4‐week, 45.5%: 8‐week) were considerably higher than in Groups I (0%: 4‐week, 0%: 8‐week), II (0%: 4‐week, 15.0%: 8‐week), and III (16.7%: 4‐week, 30.0%: 8‐week). These results demonstrated the enhancement of ExCNF bone fusion effects by BMP in a rat spinal fusion model. Our results suggest that the enhancement of ExCNFs effects by BMP makes this combination a possible attractive therapy for spinal fusion surgeries. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2892–2900, 2018.