Fluorescence imaging through dynamic scattering media with speckle-encoded ultrasound-modulated light correlation
Fluorescence imaging through dynamic scattering media with speckle-encoded ultrasound-modulated light correlation
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DOI：
10.1038/s41566-020-0630-0
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发表时间：
2020-05-11
期刊：
影响因子：
35
通讯作者：
Yang, Changhuei
中科院分区：
文献类型：
--
作者：
Ruan, Haowen;Liu, Yan;Yang, Changhuei
荧光成像是生物医学研究中不可缺少的一部分，但通过动态散射样品进行成像仍然具有挑战性。结合超声波和光的技术，例如超声波辅助的波前整形，使得通过散射介质的荧光成像成为可能。然而，由于缺乏快速的解决方案来对抗动态组织的快速散斑去相关性，这些技术在体内的应用一直受到阻碍。在这里，我们报告了一种超声波使能的光学成像方法，它利用动态性质来执行成像。该方法利用来自样本内相同位置的动态散斑编码荧光和超声调制光信号之间的相关性。我们以更高的分辨率成像荧光目标
Fluorescence imaging is indispensable to biomedical research, and yet it remains challenging to image through dynamic scattering samples. Techniques that combine ultrasound and light as exemplified by ultrasound-assisted wavefront shaping have enabled fluorescence imaging through scattering media. However, the translation of these techniques into in vivo applications has been hindered by the lack of high-speed solutions to counter the fast speckle decorrelation of dynamic tissue. Here, we report an ultrasound-enabled optical imaging method that instead leverages the dynamic nature to perform imaging. The method utilizes the correlation between the dynamic speckle-encoded fluorescence and ultrasound-modulated light signal that originate from the same location within a sample. We image fluorescent targets with an improved resolution of