A MONOCLONAL-ANTIBODY THAT APPEARS TO RECOGNIZE THE RECEPTOR FOR HUMAN T-CELL GROWTH-FACTOR - PARTIAL CHARACTERIZATION OF THE RECEPTOR
A MONOCLONAL-ANTIBODY THAT APPEARS TO RECOGNIZE THE RECEPTOR FOR HUMAN T-CELL GROWTH-FACTOR - PARTIAL CHARACTERIZATION OF THE RECEPTOR
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DOI：
10.1038/300267a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
GREENE, WC
中科院分区：
文献类型：
--
作者：
LEONARD, WJ;DEPPER, JM;GREENE, WC
T细胞生长因子（TCGF或白细胞介素-2）是一种分子量为15，000（参考文献1）的诱导型糖蛋白激素，由T淋巴细胞在抗原或有丝分裂原活化后合成和分泌2，3。TCGF是抗原消除后T细胞增殖和扩增所必需的4，5，并在体外长期培养中维持它们6 -9。人免疫应答的完全表达需要诱导TCGF合成和形成特异性TCGF膜受体10，11。结合TCGF的单克隆抗体已被证实12，13。相比之下，对TCGF膜受体特异的抗体尚未被鉴定，受体也未被表征。我们已经制备了一种单克隆抗体，称为抗-Tac 14，15，它似乎结合到TCGF的人膜受体。为了支持这一点，我们现在证明，抗Tac抑制TCGF诱导的T细胞增殖，并阻断放射性标记的TCGF与来自克隆的人连续T细胞系的细胞的结合。我们还部分纯化了推定的TCGF受体，并对其进行了表征。该受体是一种糖蛋白，分子量（Mr）为47，000 - 53，000。
T-cell growth factor (TCGF or interleukin-2) is an inducible glycoprotein hormone of molecular weight 15,000 (ref. 1) synthesized and secreted by T lymphocytes following activation with antigen or mitogen2,3. TCGF is required for proliferation and expansion of T cells following antigen encounter4,5and to maintain them in long-term culturein vitro6–9. Full expression of the human immune response requires both the induction of TCGF synthesis and the formation of specific TCGF membrane receptors10,11. Monoclonal antibodies binding TCGF have been prepared12,13. In contrast, antibodies specific for the TCGF membrane receptor have not been identified, nor has the receptor been characterized. We have prepared a monoclonal antibody, termed anti-Tac14,15, which appears to bind to the human membrane receptor for TCGF. In support of this, we now demonstrate that anti-Tac suppresses TCGF induced proliferation of T cells and blocks binding of radiolabelled TCGF to cells from a cloned human continuous T-cell line. Also we have partially purified and characterized the putative TCGF receptor. This receptor is a glycoprotein with a molecular weight (Mr) of 47,000–53,000.