Honey bee symbiont buffers larvae against nutritional stress and supplements lysine
Honey bee symbiont buffers larvae against nutritional stress and supplements lysine
复制标题
DOI：
10.1038/s41396-022-01268-x
复制
发表时间：
2022-06
期刊：
影响因子：
--
通讯作者：
Audrey J. Parish;D. W. Rice;Vicki M. Tanquary;Jason M. Tennessen;I. G. Newton
中科院分区：
文献类型：
--
作者：
Audrey J. Parish;D. W. Rice;Vicki M. Tanquary;Jason M. Tennessen;I. G. Newton
近年来，蜜蜂遭受了巨大的损失，主要是由于营养不良造成的多重压力。营养压力尤其会伤害幼虫，它们长大后无法满足群体的需求。在这项研究中，我们的特点的代谢能力的蜜蜂幼虫相关的细菌，蜂（原Parasaclobacter apium），其对幼虫的营养恢复力的影响。我们发现B。蜜蜂是唯一与幼虫相关的细菌，可以承受抗微生物幼虫饮食。此外，我们还发现B.蜜蜂能合成所有必需氨基酸，并主要通过提供必需氨基酸赖氨酸显著改变合成幼虫饲料的氨基酸含量。基因的获得/损失的分析表明，在最近获得了四个氨基酸转运蛋白B。蜜蜂祖先此外，转运蛋白LysE在所有测序的B. apis。最后，我们测试了B的影响。蜜蜂发育中的蜜蜂幼虫受到营养胁迫，发现幼虫补充B.尽管营养有限，但apisare支持减少体重。总之，这些数据表明了B的新作用。蜜蜂是蜜蜂幼虫的营养共生者。
Honey bees have suffered dramatic losses in recent years, largely due to multiple stressors underpinned by poor nutrition . Nutritional stress especially harms larvae, who mature into workers unable to meet the needs of their colony . In this study, we characterize the metabolic capabilities of a honey bee larvae-associated bacterium,Bombella apis(formerlyParasaccharibacter apium), and its effects on the nutritional resilience of larvae. We found thatB. apisis the only bacterium associated with larvae that can withstand the antimicrobial larval diet. Further, we found thatB. apiscan synthesize all essential amino acids and significantly alters the amino acid content of synthetic larval diet, largely by supplying the essential amino acid lysine. Analyses of gene gain/loss across the phylogeny suggest that four amino acid transporters were gained in recentB. apisancestors. In addition, the transporter LysE is conserved across all sequenced strains ofB. apis. Finally, we tested the impact ofB. apison developing honey bee larvae subjected to nutritional stress and found that larvae supplemented withB. apisare bolstered against mass reduction despite limited nutrition. Together, these data suggest a novel role ofB. apisas a nutritional mutualist of honey bee larvae.