Major changes in microbial diversity and community composition across gut sections of a juvenile Panchlora cockroach.
Major changes in microbial diversity and community composition across gut sections of a juvenile Panchlora cockroach.
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DOI：
10.1371/journal.pone.0177189
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Kolter R
中科院分区：
文献类型：
--
作者：
Gontang EA;Aylward FO;Carlos C;Glavina Del Rio T;Chovatia M;Fern A;Lo CC;Malfatti SA;Tringe SG;Currie CR;Kolter R
对肠道微生物组的研究揭示了这些群落的多样性和遗传内容，并帮助我们了解宿主相关微生物如何影响宿主的生理、行为和健康。尽管肠道微生物对后生动物很重要，但我们对消化道多样性和组成变化以及居民群落来源的了解仍然有限。在这里，我们利用群落宏基因组学和 16S rRNA 基因测序，评估了幼年全氯蟑螂前肠、中肠和后肠的微生物群落多样性和编码潜力，这种蟑螂生活在切叶蚁物种 Atta colombica 的垃圾堆中。我们发现 Panchora sp. 的三个肠道部分的微生物群落结构和编码潜力发生了显着变化，并且通过与之前生成的蟑螂食物来源和生态位的宏基因组进行比较，我们揭示了微生物群落组成的这种变化受到 Panchora sp. 所在生态系统的影响。发生。前肠由可能源自蚂蚁共生真菌花园的微生物组成，而中肠和后肠则由可能是蟑螂特有的微生物群落组成。与哺乳动物系统类似，中肠和后肠似乎以具有多糖降解能力的厚壁菌门和拟杆菌门为主，这表明它们可能有助于膳食植物材料的降解。我们的工作强调了整个肠道微生物组群落变化的重要性，并强调了支撑后生动物共生微生物群落结构和功能的生态因素。
Investigations of gut microbiomes have shed light on the diversity and genetic content of these communities, and helped shape our understanding of how host-associated microorganisms influence host physiology, behavior, and health. Despite the importance of gut microbes to metazoans, our understanding of the changes in diversity and composition across the alimentary tract, and the source of the resident community are limited. Here, using community metagenomics and 16S rRNA gene sequencing, we assess microbial community diversity and coding potential in the foregut, midgut, and hindgut of a juvenile Panchlora cockroach, which resides in the refuse piles of the leaf-cutter ant species Atta colombica. We found a significant shift in the microbial community structure and coding potential throughout the three gut sections of Panchlora sp., and through comparison with previously generated metagenomes of the cockroach’s food source and niche, we reveal that this shift in microbial community composition is influenced by the ecosystems in which Panchlora sp. occurs. While the foregut is composed of microbes that likely originate from the symbiotic fungus gardens of the ants, the midgut and hindgut are composed of a microbial community that is likely cockroach-specific. Analogous to mammalian systems, the midgut and hindgut appear to be dominated by Firmicutes and Bacteroidetes with the capacity for polysaccharide degradation, suggesting they may assist in the degradation of dietary plant material. Our work underscores the prominence of community changes throughout gut microbiomes and highlights ecological factors that underpin the structure and function of the symbiotic microbial communities of metazoans.