PROLIFERATIVE ACTIVITY OF CELLS IN THE SYNOVIUM AS DEMONSTRATED BY A MONOCLONAL-ANTIBODY, KI67
PROLIFERATIVE ACTIVITY OF CELLS IN THE SYNOVIUM AS DEMONSTRATED BY A MONOCLONAL-ANTIBODY, KI67
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DOI：
10.1007/bf00541375
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发表时间：
1987-01-01
影响因子：
4
通讯作者：
REVELL, PA
中科院分区：
文献类型：
--
作者：
LALOR, PA;MAPP, PI;REVELL, PA
已经使用单克隆抗体Ki67在类风湿性关节炎（RA）和骨关节炎（OA）关节的滑膜中寻找增殖细胞的存在，Ki67标记除了Go之外存在于细胞周期的所有阶段中的核抗原。应用间接免疫过氧化物酶技术对21例RA和14例OA患者的滑膜进行了研究。对18例RA和17例OA滑膜进行双重染色，同时用免疫碱性磷酸酶法标记溶菌酶（溶菌酶），用间接免疫过氧化物酶法标记Ki 67。大多数RA和OA滑膜显示内膜细胞层中不存在Ki67阳性细胞。3例RA和4例OA滑膜在整个内膜层中显示不超过10个增殖细胞。当进行双标记时，获得了类似的结果。8例RA和6例OA滑膜显示内膜层中偶尔存在Ki67阳性细胞。测量每个组织切片的内膜细胞总数，并将增殖指数计算为显示Ki67阳性染色的内膜层中总细胞的百分比。这在0.03%和0.0033%之间变化（在1：2800和1：30000细胞之间）。相比之下，在RA滑膜中的淋巴细胞浸润和血管周围以及在OA病例中的滑膜浸润（如果存在）中存在大量Ki67阳性细胞。结果进一步支持了滑膜内膜细胞层主要由巨噬细胞和一些成纤维细胞组成的观点，巨噬细胞不是通过细胞分裂（增生）局部产生的，而是从下面的滑膜血管中迁移出来的。
The presence of proliferating cells has been sought in the synovium of rheumatoid arthritic (RA) and osteoarthritic (OA) joints using the monoclonal antibody Ki67, which marks a nuclear antigen present in all stages of the cell cycle apart from Go. Synovia were studied from 21 RA and 14 OA cases using the indirect immunoperoxidase technique. Double-staining was performed on 18 RA and 17 OA synovia with the simultaneous labelling of lysozyme (muramidase) by the immuno-alkaline phosphatase method and with Ki67 by the indirect immunoperoxidase method. Nost of the RA and OA synovia showed an absence of Ki67-positive cells in the intimal cell layer. Three RA and four OA synovia showed no more than ten proliferating cells in the whole of the intimal layer examined. Similar results were obtained when double-labelling was performed. Eight RA and six OA synovia showed the presence of occasional Ki67-positive cells in the intimal layer. The total number of intimal cells was measured for each histological section, and the proliferation index calculated as the percentage of total cells in the intimal layer showing Ki67-positive staining. This varied between 0.03% and 0.0033% (between 1:2800 and 1:30 000 cells). In contrast, there were plentiful Ki67-positive cells present in the lymphocytic infiltrate and around blood vessels in the RA synovia and in the synovial infiltrate, where present, in the OA cases. The results provide further support for the suggestion that the intimal cell layer of the synovium is predominantly composed of macrophages, with some fibroblasts, and that the macrophages have not arisen locally bu cell division (hyperplasia) but have migrated from the underlying synovial blood vessels.